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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [3]
- Chromatin Immunoprecipitation [2]
- Other assay [1]
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- Product number
- PA5-77911 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- C/EBP alpha Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Positive Control: THP-1, HL-60, C2C12, HepG2, NB4, U937, THP-1, MV4-11
- Concentration
- 0.59 mg/mL
Submitted references A novel lncRNA BADLNCR1 inhibits bovine adipogenesis by repressing GLRX5 expression.
Cai H, Li M, Jian W, Song C, Huang Y, Lan X, Lei C, Chen H
Journal of cellular and molecular medicine 2020 Jul;24(13):7175-7186
Journal of cellular and molecular medicine 2020 Jul;24(13):7175-7186
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of C/EBP alpha in whole cell lysate using 30 µg of protein. Samples were separated with 10% SDS-PAGE and incubated with C/EBP alpha polyclonal antibody (Product # PA5-77911) using a dilution of 1:500 followed by HRP-conjugated anti-rabbit IgG.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of C/EBP alpha in whole cell lysate using 30 µg of protein. Samples were separated with 10% SDS-PAGE and incubated with C/EBP alpha polyclonal antibody (Product # PA5-77911) using a dilution of 1:500 followed by HRP-conjugated anti-mouse IgG.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using C/EBP alpha Polyclonal Antibody (Product # PA5-77911). Whole cell extract (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with C/EBP alpha Polyclonal Antibody (Product # PA5-77911) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Chromatin immunoprecipitation analysis of C/EBP alpha in A549 chromatin extract using 5 µg of sample. Immunoprecipitation was performed using C/EBP alpha polyclonal antibody (Product # PA5-77911). Analysis of the precipitated DNA was done by PCR using primer targeting CAMP promoter.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Cross-linked ChIP was performed with A549 chromatin extract and 5 µg of either control rabbit IgG or C/EBP alpha Polyclonal Antibody (Product # PA5-77911). The precipitated DNA was detected by PCR with primer set targeting to CAMP promoter.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- C/EBP alpha antibody immunoprecipitates C/EBP alpha protein in IP experiments. IP Sample: HepG2 whole cell lysate/extract A : Control with 3 µg of pre-immune rabbit IgG B : Immunoprecipitation of C/EBP alpha by 3 µg of C/EBP alpha antibody (Product # PA5-77911) 10% SDS-PAGE The immunoprecipitated C/EBP alpha protein was detected by C/EBP alpha antibody (Product # PA5-77911) diluted at 1:500. Anti-rabbit IgG (HRP) was used as a secondary reagent.
