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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Immunoprecipitation [1]
- Chromatin Immunoprecipitation [3]
- Other assay [1]
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- Product number
- PA5-77911 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- C/EBP alpha Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Positive Control: THP-1, HL-60, C2C12, HepG2, NB4, U937, THP-1, MV4-11 Predicted Reactivity: Rat (100%), Sheep (100%), Bovine (100%) Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.59 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references A novel lncRNA BADLNCR1 inhibits bovine adipogenesis by repressing GLRX5 expression.
Cai H, Li M, Jian W, Song C, Huang Y, Lan X, Lei C, Chen H
Journal of cellular and molecular medicine 2020 Jul;24(13):7175-7186
Journal of cellular and molecular medicine 2020 Jul;24(13):7175-7186
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- C/EBP alpha antibody immunoprecipitates C/EBP alpha protein in IP experiments. IP Sample: HepG2 whole cell lysate/extract A : Control with 3 µg of pre-immune rabbit IgG B : Immunoprecipitation of C/EBP alpha by 3 µg of C/EBP alpha antibody (Product # PA5-77911) 10% SDS-PAGE The immunoprecipitated C/EBP alpha protein was detected by C/EBP alpha antibody (Product # PA5-77911) diluted at 1:500. Anti-rabbit IgG (HRP) was used as a secondary reagent.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Chromatin immunoprecipitation analysis of C/EBP alpha in A549 chromatin extract using 5 µg of sample. Immunoprecipitation was performed using C/EBP alpha polyclonal antibody (Product # PA5-77911). Analysis of the precipitated DNA was done by PCR using primer targeting CAMP promoter.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Cross-linked ChIP was performed with A549 chromatin extract and 5 µg of either control rabbit IgG or C/EBP alpha Polyclonal Antibody (Product # PA5-77911). The precipitated DNA was detected by PCR with primer set targeting to CAMP promoter.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Cross-linked ChIP was performed with A549 chromatin extract and 5 µg of either control rabbit IgG or C/EBP alpha Polyclonal Antibody (Product # PA5-77911). The precipitated DNA was detected by PCR with primer set targeting to CAMP promoter.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- C/EBP alpha antibody immunoprecipitates C/EBP alpha protein in IP experiments. IP Sample: HepG2 whole cell lysate/extract A : Control with 3 µg of pre-immune rabbit IgG B : Immunoprecipitation of C/EBP alpha by 3 µg of C/EBP alpha antibody (Product # PA5-77911) 10% SDS-PAGE The immunoprecipitated C/EBP alpha protein was detected by C/EBP alpha antibody (Product # PA5-77911) diluted at 1:500. Anti-rabbit IgG (HRP) was used as a secondary reagent.
