Antibody data
- Antibody Data
- Antigen structure
- References [7]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- A00386-1 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-CEBP Alpha/CEBPA Antibody Picoband™
- Antibody type
- Polyclonal
- Description
- Rabbit IgG polyclonal antibody for CEBP Alpha/CEBPA detection. Tested with WB, ICC/IF, FCM, Direct ELISA in Human;Mouse;Rat.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Vial size
- 100μg/vial
- Concentration
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
- Handling
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Submitted references An intronic enhancer of Cebpa regulates adipocyte differentiation and adipose tissue development via long-range loop formation.
Quinacrine attenuates diet-induced obesity by inhibiting adipogenesis via activation of AMPK signaling.
The effects of Formoterol in preventing adipogenesis and obesity are mediated by PPARγ/C/EBPα axis and AMPK/PGC-1α pathway.
β-Cypermethrin Alleviated the Inhibitory Effect of Medium from RAW 264.7 Cells on 3T3-L1 Cell Maturation into Adipocytes.
β -Cypermethrin promotes the adipogenesis of 3T3-L1 cells via inducing autophagy and shaping an adipogenesis-friendly microenvironment.
Circular RNA CDR1as promotes adipogenic and suppresses osteogenic differentiation of BMSCs in steroid-induced osteonecrosis of the femoral head.
LncRNA expression profiling of BMSCs in osteonecrosis of the femoral head associated with increased adipogenic and decreased osteogenic differentiation.
Li X, Zeng S, Chen L, Zhang Y, Li X, Zhang B, Su D, Du Q, Zhang J, Wang H, Zhong Z, Zhang J, Li P, Jiang A, Long K, Li M, Ge L
Cell proliferation 2024 Mar;57(3):e13552
Cell proliferation 2024 Mar;57(3):e13552
Quinacrine attenuates diet-induced obesity by inhibiting adipogenesis via activation of AMPK signaling.
Li X, Zhou J, Hu Z, Han L, Zhao W, Wu T, Li C, Liu J, Kang J, Chen X
European journal of pharmacology 2023 Jan 5;938:175432
European journal of pharmacology 2023 Jan 5;938:175432
The effects of Formoterol in preventing adipogenesis and obesity are mediated by PPARγ/C/EBPα axis and AMPK/PGC-1α pathway.
Zhang X, Che L, Shan J, Wang Y, Jia Y, Wu H
Bioscience, biotechnology, and biochemistry 2022 Jul 4;
Bioscience, biotechnology, and biochemistry 2022 Jul 4;
β-Cypermethrin Alleviated the Inhibitory Effect of Medium from RAW 264.7 Cells on 3T3-L1 Cell Maturation into Adipocytes.
He B, Wang X, Jin X, Xue Z, Ni Y, Zhu J, Wang C, Jin Y, Fu Z
Lipids 2020 May;55(3):251-260
Lipids 2020 May;55(3):251-260
β -Cypermethrin promotes the adipogenesis of 3T3-L1 cells via inducing autophagy and shaping an adipogenesis-friendly microenvironment.
He B, Wang X, Jin X, Xue Z, Zhu J, Wang C, Jin Y, Fu Z
Acta biochimica et biophysica Sinica 2020 Aug 5;52(8):821-831
Acta biochimica et biophysica Sinica 2020 Aug 5;52(8):821-831
Circular RNA CDR1as promotes adipogenic and suppresses osteogenic differentiation of BMSCs in steroid-induced osteonecrosis of the femoral head.
Chen G, Wang Q, Li Z, Yang Q, Liu Y, Du Z, Zhang G, Song Y
Bone 2020 Apr;133:115258
Bone 2020 Apr;133:115258
LncRNA expression profiling of BMSCs in osteonecrosis of the femoral head associated with increased adipogenic and decreased osteogenic differentiation.
Wang Q, Yang Q, Chen G, Du Z, Ren M, Wang A, Zhao H, Li Z, Zhang G, Song Y
Scientific reports 2018 Jun 14;8(1):9127
Scientific reports 2018 Jun 14;8(1):9127
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Supportive validation
- Submitted by
- Boster Biological Technology (provider)
- Main image
- Experimental details
- Western blot analysis of CEBPA using anti-CEBPA antibody (A00386-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human U-937 whole cell lysates, Lane 2: human HepG2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CEBPA antigen affinity purified polyclonal antibody (Catalog # A00386-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CEBPA at approximately 42,30KD. The expected band size for CEBPA is at 42,30KD.
- Additional image