PA5-21651

antibody from Invitrogen Antibodies

Targeting: ANXA7 ANX7

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-21651

Provider

Invitrogen Antibodies

Product name

Annexin A7 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: 293T, A431, HeLa, HepG2.

Concentration

0.28 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Annexin A7 Polyclonal Antibody detects Annexin VII protein by western blot analysis. A. 30 µg 293T whole cell extract. B. 30 µg A431 whole cell extract. C. 30 µg HeLa whole cell extract. D. 30 µg HepG2 whole cell extract.10 % SDS-PAGE. Annexin A7 Polyclonal Antibody (Product # PA5-21651) dilution: 1:1,000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Annexin A7 Polyclonal Antibody detects Annexin VII protein by western blot analysis. A. 30 µg 293T whole cell extract. B. 30 µg A431 whole cell extract. C. 30 µg HeLa whole cell extract. D. 30 µg HepG2 whole cell extract.10 % SDS-PAGE. Annexin A7 Polyclonal Antibody (Product # PA5-21651) dilution: 1:1,000.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Annexin A7 Polyclonal Antibody detects ANXA7 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: ANXA7 protein stained by Annexin A7 Polyclonal Antibody (Product # PA5-21651) diluted at 1:500. Blue: Hoechst 33343 staining.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Annexin A7 Polyclonal Antibody detects Annexin VII protein at cytosol on human gastric carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human gastric carcinoma. Annexin A7 Polyclonal Antibody (Product # PA5-21651) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.