Antibody data
- Antibody Data
- Antigen structure
- References [14]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- 15-0869-41 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-CD86 (B7-2) Monoclonal Antibody (IT2.2), PE-Cyanine5, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The IT2.2 monoclonal antibody reacts with human CD86, an ~80 kDa surface receptor also known as B7-2. CD86 and CD80 are members of the B7 family of costimulatory molecules. CD86 is expressed at low levels on B cells, macrophages, and dendritic cells and is upregulated on B cells through a variety of surface stimuli including the BCR complex, CD40 and some cytokine receptors. In addition to CD80 (B7-1), CD86 is a counter-receptor for the T cell surface molecules CD28 and CD152 (CTLA-4). The interaction of CD86 with its ligands plays a critical role in T-B crosstalk, T cell costimulation, autoantibody production and Th2-mediated Ig production. The kinetics of upregulation of CD86 upon stimulation supports its major contribution during the primary phase of an immune response. Applications Reported: The IT2.2 antibody has been reported for use in flow cytometric analysis. Applications Tested: This IT2.2 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 488-561 nm; Emission: 667 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- IT2.2
- Vial size
- 25 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Ginsenoside Rg3 Mitigates Atherosclerosis Progression in Diabetic apoE-/- Mice by Skewing Macrophages to the M2 Phenotype.
AG490 reverses phenotypic alteration of dendritic cells by bladder cancer cells.
Photodynamic therapy reduces the inhibitory effect of osteosarcoma cells on dendritic cells by upregulating HSP70.
Abnormal phenotypic features of IgM+B cell subsets in patients with chronic hepatitis C virus infection.
Leishmania infantum and Leishmania braziliensis: Differences and Similarities to Evade the Innate Immune System.
MicroRNA-720 suppresses M2 macrophage polarization by targeting GATA3.
Omental implantation of BOECs in hemophilia dogs results in circulating FVIII antigen and a complex immune response.
Naïve and memory B cells in the rhesus macaque can be differentiated by surface expression of CD27 and have differential responses to CD40 ligation.
Effects of NOD-like receptors in human B lymphocytes and crosstalk between NOD1/NOD2 and Toll-like receptors.
Linking innate and adaptive immunity: human Vgamma9Vdelta2 T cells enhance CD40 expression and HMGB-1 secretion.
Mycobacterium tuberculosis-induced gamma interferon production by natural killer cells requires cross talk with antigen-presenting cells involving Toll-like receptors 2 and 4 and the mannose receptor in tuberculous pleurisy.
Functional expression of a costimulatory B7.2 (CD86) protein on human salivary gland epithelial cells that interacts with the CD28 receptor, but has reduced binding to CTLA4.
CD86 (B7-2) on human B cells. A functional role in proliferation and selective differentiation into IgE- and IgG4-producing cells.
B70 antigen is a second ligand for CTLA-4 and CD28.
Guo M, Xiao J, Sheng X, Zhang X, Tie Y, Wang L, Zhao L, Ji X
Frontiers in pharmacology 2018;9:464
Frontiers in pharmacology 2018;9:464
AG490 reverses phenotypic alteration of dendritic cells by bladder cancer cells.
Xiu W, Ma J, Lei T, Zhang M
Oncology letters 2018 Sep;16(3):2851-2856
Oncology letters 2018 Sep;16(3):2851-2856
Photodynamic therapy reduces the inhibitory effect of osteosarcoma cells on dendritic cells by upregulating HSP70.
Zhang F, Zhu Y, Fan G, Hu S
Oncology letters 2018 Oct;16(4):5034-5040
Oncology letters 2018 Oct;16(4):5034-5040
Abnormal phenotypic features of IgM+B cell subsets in patients with chronic hepatitis C virus infection.
Kong F, Feng B, Zhang H, Rao H, Wang J, Cong X, Wei L
Experimental and therapeutic medicine 2017 Aug;14(2):1846-1852
Experimental and therapeutic medicine 2017 Aug;14(2):1846-1852
Leishmania infantum and Leishmania braziliensis: Differences and Similarities to Evade the Innate Immune System.
Falcão Sde A, Jaramillo TM, Ferreira LG, Bernardes DM, Santana JM, Favali CB
Frontiers in immunology 2016;7:287
Frontiers in immunology 2016;7:287
MicroRNA-720 suppresses M2 macrophage polarization by targeting GATA3.
Zhong Y, Yi C
Bioscience reports 2016 Aug;36(4)
Bioscience reports 2016 Aug;36(4)
Omental implantation of BOECs in hemophilia dogs results in circulating FVIII antigen and a complex immune response.
Ozelo MC, Vidal B, Brown C, Notley C, Hegadorn C, Webster S, Harpell L, Ahlin J, Winterborn A, Handforth J, Arruda VR, Hough C, Lillicrap D
Blood 2014 Jun 26;123(26):4045-53
Blood 2014 Jun 26;123(26):4045-53
Naïve and memory B cells in the rhesus macaque can be differentiated by surface expression of CD27 and have differential responses to CD40 ligation.
Kuhrt D, Faith S, Hattemer A, Leone A, Sodora D, Picker L, Borghesi L, Cole KS
Journal of immunological methods 2011 Jan 5;363(2):166-76
Journal of immunological methods 2011 Jan 5;363(2):166-76
Effects of NOD-like receptors in human B lymphocytes and crosstalk between NOD1/NOD2 and Toll-like receptors.
Petterson T, Jendholm J, Månsson A, Bjartell A, Riesbeck K, Cardell LO
Journal of leukocyte biology 2011 Feb;89(2):177-87
Journal of leukocyte biology 2011 Feb;89(2):177-87
Linking innate and adaptive immunity: human Vgamma9Vdelta2 T cells enhance CD40 expression and HMGB-1 secretion.
Kalyan S, Chow AW
Mediators of inflammation 2009;2009:819408
Mediators of inflammation 2009;2009:819408
Mycobacterium tuberculosis-induced gamma interferon production by natural killer cells requires cross talk with antigen-presenting cells involving Toll-like receptors 2 and 4 and the mannose receptor in tuberculous pleurisy.
Schierloh P, Yokobori N, Alemán M, Landoni V, Geffner L, Musella RM, Castagnino J, Baldini M, Abbate E, de la Barrera SS, Sasiain MC
Infection and immunity 2007 Nov;75(11):5325-37
Infection and immunity 2007 Nov;75(11):5325-37
Functional expression of a costimulatory B7.2 (CD86) protein on human salivary gland epithelial cells that interacts with the CD28 receptor, but has reduced binding to CTLA4.
Kapsogeorgou EK, Moutsopoulos HM, Manoussakis MN
Journal of immunology (Baltimore, Md. : 1950) 2001 Mar 1;166(5):3107-13
Journal of immunology (Baltimore, Md. : 1950) 2001 Mar 1;166(5):3107-13
CD86 (B7-2) on human B cells. A functional role in proliferation and selective differentiation into IgE- and IgG4-producing cells.
Jeannin P, Delneste Y, Lecoanet-Henchoz S, Gauchat JF, Ellis J, Bonnefoy JY
The Journal of biological chemistry 1997 Jun 20;272(25):15613-9
The Journal of biological chemistry 1997 Jun 20;272(25):15613-9
B70 antigen is a second ligand for CTLA-4 and CD28.
Azuma M, Ito D, Yagita H, Okumura K, Phillips JH, Lanier LL, Somoza C
Nature 1993 Nov 4;366(6450):76-9
Nature 1993 Nov 4;366(6450):76-9
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Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of normal human peripheral blood cells with Mouse IgG2b Isotype Control PE-Cyanine5 (Product # 15-4732-42) (blue histogram) or Anti-Human CD86 (B7-2) PE-Cyanine5 (purple histogram).Cells in the monocyte gate were used for analysis.