HPA021213
SMARCC2 antibody from Atlas Antibodies
BAF170, CRACC2, Rsc8
Validated within the Antibodypedia Validation Initative
Antibody data
- Product number
- HPA021213
- Provider
- Atlas Antibodies
- Proper citation
- Atlas Antibodies Cat#HPA021213, RRID:AB_1857275
- Product name
- Anti-SMARCC2
- Provider product page
- Atlas Antibodies - HPA021213
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Antigen sequence
AFGLESSGIAGTTSDEPERIEESGNDEARVEGQAT
DEKKEPKEPREGGGAIEEEAKEKTSEAPKKDEEKG
KEGDSEKESEKSDGDPIVDPEKEKEPKEGQEEVLK
EVVESEGERKTKVERDIGEGNLSTAA
- Isotype
- IgG
- Vial size
- 100 µl
- Storage
- Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Supportive validation
- Submitted by
-
Atlas Antibodies (provider)
- Main image
- Experimental details
- Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)
Supportive validation
- Submitted by
-
Atlas Antibodies (provider)
- Main image
- Experimental details
- Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11Lane 2: Human cell line RT-4Lane 3: Human cell line U-251MG sp
Supportive validation
- Submitted by
-
Atlas Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
- Sample type
- HUMAN
Enhanced validation
- Submitted by
-
55af80e3e0991
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Confocal images of immunofluorescently stained human U-2 OS cells.The protein SMARCC2 is shown in green. The image to the left show cells transfected with control siRNA and the image to the right show cells where SMARCC2 has been downregulated with specific siRNA.
- Sample type
- U-2 OS cells
- Primary Ab dilution
- 1:25
- Secondary Ab
- Secondary Ab
- Secondary Ab dilution
- 1:800
- Knockdown/Genetic Approaches Application
- Immunocytochemistry
- KD Reagent Type
- siRNA
- Downregulation
- >50%
- KD Reagent Provider
- Thermo Fisher Scientific
- KD Reagent Prod no
- s13149
- KD Reagent Prod page
- KD Reagent Prod page
- KD Reagent Prod no 2
- s13150
- KD Reagent Page 2
- KD Reagent Page 2
- KD Reagent Prod no 3
- s13148
- KD Reagent Page 3
- KD Reagent Page 3
- Antibody Lot Number
- R10644
- Appendix
- 55b5faabae98f.pdf
- Show more
Supportive validation
- Submitted by
-
Atlas Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of human stomach shows strong nuclear positivity in glandular cells.
- Sample type
- HUMAN
Supportive data in Antibodypedia
Enhanced validation
