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Western blot
Immunoprecipitation
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunohistochemistry [1]
- Other assay [2]
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- Product number
- A302-242A - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- BAP1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- 4° C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human BAP1 in FFPE prostate carcinoma by IHC. Antibody: Rabbit anti-BAP1 antibody (Product # A302-242A; lot 2). Secondary: HRP-conjugated goat anti-rabbit IgG (Product # A120-501P). Substrate: DAB.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human BAP1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Hela cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-BAP1 antibody (Product # A302-242A; A302-242A Lot 2) used for IP at 6 µg per reaction. BAP1 was also immunoprecipitated by a previous lot of this antibody (A302-242A Lot 1) and rabbit anti-BAP1 antibody (Product # A302-243A). For blotting immunoprecipitated BAP1 (Product # A302-243A) was used at 1 µg/mL. Detection: Chemiluminescence with an exposure time of 10 seconds.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human BAP1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-BAP1 antibody A302-242A used for IP at 10 µg/mg lysate. BAP1 was also immunoprecipitated by rabbit anti-BAP1 antibody A302-243A, which recognizes a downstream epitope. For blotting immunoprecipitated BAP1, A302-243A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
