MA1-46087

antibody from Invitrogen Antibodies

Targeting: BAP1 hucep-6, KIAA0272, UCHL2

Antibody data

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Validation data

Product number: MA1-46087 - Provider product page
Provider: Invitrogen Antibodies
Product name: BAP1 Monoclonal Antibody (1G8)
Antibody type: Monoclonal
Antigen: Purifed from natural sources
Description: Suggested positive control: HeLa whole cell lysate, antigen standard for BAP1 (transient overexpression lysate).
Reactivity: Human
Host: Mouse
Isotype: IgG
Antibody clone number: 1G8
Vial size: 100 µL
Concentration: 1 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

BAP1 protein structure - MA1-46087 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of BAP1 in HeLa whole cell extracts using a monoclonal antibody (Product # MA1-46087).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of BAP1 in HeLa WCE. Sample was incubated in BAP1 monoclonal antibody (Product # MA1-46087).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Knockdown of Ubiquitin carboxyl-terminal hydrolase BAP1 was achieved by transfecting THP-1 with Ubiquitin carboxyl-terminal hydrolase BAP1 specific siRNAs (Silencer® select Product # S15820, S15822). Western Blot analysis (Fig. a) was performed using Whole cell extracts from the Ubiquitin carboxyl-terminal hydrolase BAP1 knockdown cells (lane 3), non-targeting scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The Blot was probed with BAP1 Monoclonal Antibody (1G8) (Product # MA1-46087, 1:2000 ) and Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:20000). Densitometric analysis of this western Blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to Ubiquitin carboxyl-terminal hydrolase BAP1.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot was performed using Anti-BAP1 Monoclonal Antibody (1G8) (Product # MA1-46087) and a 80 kDa and 100 kDa band corresponding to Ubiquitin carboxyl-terminal hydrolase BAP1 was observed across cell lines and tissue. Whole cell extracts (40 µg lysate) of THP-1 (Lane 1), HeLa (Lane 2), DU 145 (Lane 3), NCI-H1299 (Lane 4) and Mouse Testis (Lane 5) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # LC2002) by iBlot® 2 Dry Blotting System (Product # IB21001). The Blot was probed with the primary antibody (1:2000) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:20000) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Atto Ultimate Sensitivity Substrate (Product # A38556).