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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- AF7279 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse RIAM/APBB1IP Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects recombinant human RIAM/APBB1IP in direct ELISAs. Detects human and mouse RIAM/APBB1IP in Western blots
- Reactivity
- Human, Mouse
- Host
- Sheep
- Conjugate
- Unconjugated
- Antigen sequence
Q7Z5R6- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Cutting Edge: Loss of T Cell RIAM Precludes Conjugate Formation with APC and Prevents Immune-Mediated Diabetes.
Lagarrigue F, Gertler FB, Ginsberg MH, Cantor JM
Journal of immunology (Baltimore, Md. : 1950) 2017 May 1;198(9):3410-3415
Journal of immunology (Baltimore, Md. : 1950) 2017 May 1;198(9):3410-3415
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human and Mouse RIAM/APBB1IP by Western Blot. Western blot shows lysates of THP-1 human acute monocytic leukemia cell line and EL-4 mouse lymphoblast cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human RIAM/APBB1IP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7279) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for RIAM/APBB1IP at approximately 100-110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- RIAM/APBB1IP in Wi-38 Human Cell Line. RIAM/APBB1IP was detected in immersion fixed Wi-38 human lung fibroblast cell line using Sheep Anti-Human RIAM/APBB1IP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7279) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel; Catalog # NL010) and counterstained with DAPI (blue, lower panel). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
