Antibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- A010-50 - Provider product page
- Provider
- Badrilla Ltd
- Product name
- CaMK II Phospho Threonine-286 Anti-Serum
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Ca2+/calmodulin-dependent kinase II (CaMKII) is a ubiquitous, multifunctional serine/threonine kinase involved in translating Ca2+- transient signals into cellular responses. Four separate CaMKII genes are expressed in man (α, β, γ, δ) with isoforms within each gene. CaMKII γ and δ are abundant in the heart. CaMKII is activated by Ca2+/CaM binding, and becomes activated for prolonged periods of time following autophosphorylation of Thr-286 or Thr-287 (equivalent residues in different isoforms). This antibody recognizes the active, autophosphorylated enzyme (all isoforms) and thus can measure the abundance of active CaMKII. Prolonged activation of CaMKII is associated with a variety of vascular and cardiac diseases including stroke, atrial fibrillation cardiac arrhythmia & sudden death, cardiac apoptosis, and heart failure.
- Reactivity
- Human, Rat, Bovine, Porcine, Rabbit
- Host
- Rabbit
- Antigen sequence
(M281HRQET(PO3H2)VDC289)
- Isotype
- IgG
- Vial size
- 20 µl
- Storage
- Lyophilised: 4 °C Reconstituted:-80 °C
- Handling
- Reconstitute lyophilised powder in 20 µl of 18 MΩ H2O, aliquot and store frozen at -80 °C for 1 year. Avoid freeze - thaw cycles
Submitted references Fatty acid synthase modulates homeostatic responses to myocardial stress.
A CaMKIIβ signaling pathway at the centrosome regulates dendrite patterning in the brain.
Na+/K+-ATPase inhibition by ouabain induces CaMKII-dependent apoptosis in adult rat cardiac myocytes.
Alpha-kinase anchoring protein alphaKAP interacts with SERCA2A to spatially position Ca2+/calmodulin-dependent protein kinase II and modulate phospholamban phosphorylation.
Razani B, Zhang H, Schulze PC, Schilling JD, Verbsky J, Lodhi IJ, Topkara VK, Feng C, Coleman T, Kovacs A, Kelly DP, Saffitz JE, Dorn GW 2nd, Nichols CG, Semenkovich CF
The Journal of biological chemistry 2011 Sep 2;286(35):30949-61
The Journal of biological chemistry 2011 Sep 2;286(35):30949-61
A CaMKIIβ signaling pathway at the centrosome regulates dendrite patterning in the brain.
Puram SV, Kim AH, Ikeuchi Y, Wilson-Grady JT, Merdes A, Gygi SP, Bonni A
Nature neuroscience 2011 Jul 3;14(8):973-83
Nature neuroscience 2011 Jul 3;14(8):973-83
Na+/K+-ATPase inhibition by ouabain induces CaMKII-dependent apoptosis in adult rat cardiac myocytes.
Sapia L, Palomeque J, Mattiazzi A, Petroff MV
Journal of molecular and cellular cardiology 2010 Sep;49(3):459-68
Journal of molecular and cellular cardiology 2010 Sep;49(3):459-68
Alpha-kinase anchoring protein alphaKAP interacts with SERCA2A to spatially position Ca2+/calmodulin-dependent protein kinase II and modulate phospholamban phosphorylation.
Singh P, Salih M, Tuana BS
The Journal of biological chemistry 2009 Oct 9;284(41):28212-21
The Journal of biological chemistry 2009 Oct 9;284(41):28212-21
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Supportive validation
- Submitted by
- Badrilla Ltd (provider)
- Main image
- Experimental details
- Western blot
- Sample type
- Rat brain homogenate +/- CaMKII treatment with and without immunizing peptide
- Validation comment
- Ab stains for α and β isoforms of threonine-286 phosphorylated CaMKII. Incubation with peptide blocks antibody staining
- Primary Ab dilution
- 3.513888889
- Secondary Ab dilution
- 1.430555556
- Protocol
- Protocol