Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- A010-50AP - Provider product page
- Provider
- Badrilla Ltd
- Product name
- CaMK II Phospho Threonine-286 (AP) IgG
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Ca2+/calmodulin-dependent kinase II (CaMKII) is a ubiquitous, multifunctional serine/threonine kinase involved in translating Ca2+- transient signals into cellular responses. Four separate CaMKII genes are expressed in man (α, β, γ, δ) with isoforms within each gene. CaMKII γ and δ are abundant in the heart. CaMKII is activated by Ca2+/CaM binding, and becomes activated for prolonged periods of time following autophosphorylation of Thr-286 or Thr-287 (equivalent residues in different isoforms). This antibody recognizes the active, autophosphorylated enzyme (all isoforms) and thus can measure the abundance of active CaMKII. Prolonged activation of CaMKII is associated with a variety of vascular and cardiac diseases including stroke, atrial fibrillation cardiac arrhythmia & sudden death, cardiac apoptosis, and heart failure.
- Reactivity
- Human, Rat, Bovine, Porcine, Rabbit
- Host
- Rabbit
- Antigen sequence
(M281HRQET(PO3H2)VDC289)
- Isotype
- IgG
- Vial size
- 50 µl
- Storage
- Lyophilised: 4 °C Reconstituted:-80 °C
- Handling
- Reconstitute lyophilised powder in 50 µl of 18 MΩ H2O, aliquot and store frozen at -80 °C for 1 year. Avoid freeze - thaw cycles
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Supportive validation
- Submitted by
- Badrilla Ltd (provider)
- Main image
- Experimental details
- Western blot
- Sample type
- Rat brain homogenate +/- CaMKII treatment
- Validation comment
- Ab stains for α and β isoforms of threonine-286 phosphorylated CaMKII
- Primary Ab dilution
- 0.736111111
- Secondary Ab dilution
- 1.430555556
- Protocol
- Protocol