H00000815-D01P

antibody from Abnova Corporation

Targeting: CAMK2A CAMKA, CaMKIINalpha, CaMKIIα, KIAA0968

Western blot (Supportive data in Antibodypedia)

Proximity ligation assay (Supportive data in Antibodypedia)

Antibody Data

Product number

H00000815-D01P

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00000815-D01P, RRID:AB_10550675

Product name

CAMK2A purified MaxPab rabbit polyclonal antibody (D01P)

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against a full-length human CAMK2A protein.

Antigen sequence

MATITCTRFTEEYQLFEELGKGAFSVVRRCVKVLA
GQEYAAKIINTKKLSARDHQKLEREARICRLLKHP
NIVRLHDSISEEGHHYLIFDLVTGGELFEDIVARE
YYSEADASHCIQQILEAVLHCHQMGVVHRDLKPEN
LLLASKLKGAAVKLADFGLAIEVEGEQQAWFGFAG
TPGYLSPEVLRKDPYGKPVDLWACGVILYILLVGY
PPFWDEDQHRLYKQIKAGAYDFPSPEWDTVTPEAK
DLINKMLTINPSKRITAAEALKHPWISHRSTVASC
MHRQETVDCLKKFNARRKLKGAILTTMLATRNFSG
GKSGGNKKSDGVKESSESTNTTIEDEDTKVRKQEI
IKVTEQLIEAISNGDFESYTKMCDPGMTAFEPEAL
GNLVEGLDFHRFYFENLWSRNSKPVHTTILNPHIH
LMGDESACIAYIRITQYLDAGGIPRTAQSEETRVW
HRRDGKWQIVHFHRSGAPSVLPH


Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of CAMK2A expression in transfected 293T cell line (H00000815-T01) by CAMK2A MaxPab polyclonal antibody.Lane 1: CAMK2A transfected lysate(52.69 KDa).Lane 2: Non-transfected lysate.

Proximity ligation assay

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Proximity Ligation Analysis of protein-protein interactions between CAMK2A and SMAD2. HeLa cells were stained with anti-CAMK2A rabbit purified polyclonal 1:1200 and anti-SMAD2 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Validation comment

In situ Proximity Ligation Assay (Cell)