RQ5962

antibody from NSJ Bioreagents

Targeting: CAMK2A CAMKA, CaMKIINalpha, CaMKIIα, KIAA0968

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

RQ5962

Provider

NSJ Bioreagents

Product name

CAMK II alpha Antibody / CAMK2A

Antibody type

Polyclonal

Description

This highly specific CAMK II alpha antibody is suitable for use in Western blot/Flow cytometry/Immunofluorescence/Direct ELISA applications with human, mouse and rat samples.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Vial size

100 ug

Concentration

0.5mg/ml if reconstituted with 0.2ml sterile DI water

Storage

After reconstitution, the CAMK II alpha antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of different lots of rat brain (lanes 1-2) and mouse brain (lanes 3-4) lysate with CAMK II alpha antibody. Predicted molecular weight: ~54 kDa. An additional ~65 kDa band is often observed.

Immunocytochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Immunofluorescent staining of FFPE human MCF7 cells with CAMK II alpha antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of human U-87 MG cells with CAMK II alpha antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= CAMK II alpha antibody.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of mouse ANA-1 cells with CAMK II alpha antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= CAMK II alpha antibody.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of rat NRK cells with CAMK II alpha antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= CAMK II alpha antibody.