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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [2]
- Immunohistochemistry [3]
- Flow cytometry [1]
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- Product number
- NBP1-51945 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-51945, RRID:AB_11037869
- Product name
- Goat Polyclonal CaM Kinase II alpha Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. This antibody is expected to recognize both reported isoforms (NP_057065.2
- Reactivity
- Human, Mouse, Rat
- Host
- Goat
- Antigen sequence
C-PRTAQSEETRVWHR- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references Neural Deletion of Glucose Transporter Isoform 3 Creates Distinct Postnatal and Adult Neurobehavioral Phenotypes.
Tumor necrosis factor-alpha enhances neutrophil adhesiveness: induction of vascular cell adhesion molecule-1 via activation of Akt and CaM kinase II and modifications of histone acetyltransferase and histone deacetylase 4 in human tracheal smooth muscle cells.
Shin BC, Cepeda C, Estrada-Sánchez AM, Levine MS, Hodaei L, Dai Y, Jung J, Ganguly A, Clark P, Devaskar SU
The Journal of neuroscience : the official journal of the Society for Neuroscience 2018 Oct 31;38(44):9579-9599
The Journal of neuroscience : the official journal of the Society for Neuroscience 2018 Oct 31;38(44):9579-9599
Tumor necrosis factor-alpha enhances neutrophil adhesiveness: induction of vascular cell adhesion molecule-1 via activation of Akt and CaM kinase II and modifications of histone acetyltransferase and histone deacetylase 4 in human tracheal smooth muscle cells.
Lee CW, Lin CC, Luo SF, Lee HC, Lee IT, Aird WC, Hwang TL, Yang CM
Molecular pharmacology 2008 May;73(5):1454-64
Molecular pharmacology 2008 May;73(5):1454-64
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CaM Kinase II alpha Antibody [NBP1-51945] - Staining of Human Cerebellum (A), Mouse (B) and Rat Brain (C) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CaM Kinase II alpha Antibody [NBP1-51945] - Staining of Human Cerebellum (A), Mouse (B) and Rat (C) Brain lysate (35ug protein in RIPA buffer). Detected by chemiluminescence
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: CaM Kinase II alpha Antibody [NBP1-51945] - Staining of paraffin embedded Human Brain Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: CaM Kinase II alpha Antibody [NBP1-51945] - IHC using CamKII antibody from goat in 3% PFA perfused mouse brain. 1:100 dilution clearly provides cytosolic CamK II immunoreactivity in whole brain regions, including the hippocampus (HP), cortex (CTX) and paraventricular region (PVN). Image from verified customer review.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: CaM Kinase II alpha Antibody [NBP1-51945] - Staining of paraffin embedded Human Adrenal Gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: CaM Kinase II alpha Antibody [NBP1-51945] - Analysis of paraformaldehyde fixed Kelly cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
