NB110-96869

antibody from Novus Biologicals

Targeting: CAMK2A CAMKA, CaMKIINalpha, CaMKIIα, KIAA0968

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NB110-96869

Provider

Novus Biologicals

Proper citation

Novus Cat#NB110-96869, RRID:AB_1260569

Product name

Mouse Monoclonal CaMKII alpha/beta Antibody

Antibody type

Monoclonal

Antigen

Synthetic peptide

Description

Protein G purified. This antibody is specific for a and B subunits of CaMKII only when they are phosphorylated at Thr-286/287 (in B).

Reactivity

Human, Mouse, Rat

Host

Mouse

Isotype

IgG

Vial size

0.1 mg

Concentration

1 mg/ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

References

Submitted references

MerTK signaling in macrophages promotes the synthesis of inflammation resolution mediators by suppressing CaMKII activity.

Cai B, Kasikara C, Doran AC, Ramakrishnan R, Birge RB, Tabas I
Science signaling 2018 Sep 25;11(549)

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Thioesterase-mediated control of cellular calcium homeostasis enables hepatic ER stress.

Ersoy BA, Maner-Smith KM, Li Y, Alpertunga I, Cohen DE
The Journal of clinical investigation 2018 Jan 2;128(1):141-156

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Inhibition of apolipoprotein B synthesis stimulates endoplasmic reticulum autophagy that prevents steatosis.

Conlon DM, Thomas T, Fedotova T, Hernandez-Ono A, Di Paolo G, Chan RB, Ruggles K, Gibeley S, Liu J, Ginsberg HN
The Journal of clinical investigation 2016 Oct 3;126(10):3852-3867

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Calmodulin-dependent protein kinase II/cAMP response element-binding protein/Wnt/β-catenin signaling cascade regulates angiotensin II-induced podocyte injury and albuminuria.

Jiang L, Xu L, Song Y, Li J, Mao J, Zhao AZ, He W, Yang J, Dai C
The Journal of biological chemistry 2013 Aug 9;288(32):23368-79

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: CaMKII alpha/beta [p Thr286, p Thr287] Antibody (22B1) [NB110-96869] - analysis of CaMKII and NFAT phosphorylation in ventricles of 14 day old mice over-expressing CaMK.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: CaMKII alpha/beta [p Thr286, p Thr287] Antibody (22B1) [NB110-96869] - Bouin's fixed paraffin-embedded backskin sections of transgenic mice- muscle, and hair follicle stiaing. Courtesy of Dr. Turksen, Ottawa Hospital Research Institute, Canada.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry: CaMKII alpha/beta [p Thr286, p Thr287] Antibody (22B1) [NB110-96869] - analysis using Mouse Anti-CaMKII Monoclonal Antibody, Clone 22B1 . Tissue: dissociated hippocampal neurons. Species: Rat. Fixation: Cold 4% paraformaldehyde/0.2% glutaraldehyde in 0.1M sodium phosphate buffer. Primary Antibody: Mouse Anti-CaMKII Monoclonal Antibody at 1:1000 for 12 hours at 4C. Secondary Antibody: FITC Goat Anti-Mouse IgG (green) at 1:50 for 30 minutes at RT. Magnification: 10X. Courtesy of: Mary Kennedy, Caltech.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: CaMKII alpha/beta [p Thr286, p Thr287] Antibody (22B1) [NB110-96869] - Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-CaMKII Monoclonal Antibody at 1:5000 for 12 hours at 4C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 ul for 2 minutes at RT. Magnification: 40x.