AP09284PU-N

antibody from Acris Antibodies GmbH

Targeting: CAMK2A CAMKA, CaMKIINalpha, CaMKIIα, KIAA0968

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Antibody Data

Product number

AP09284PU-N

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#AP09284PU-N, RRID:AB_2035239

Product name

anti CAMK2A (6-23)

Antibody type

Polyclonal

Antigen

Synthetic peptide corresponding to amino acids 6-23 of Human CaM Kinase II protein

Reactivity

Human, Mouse, Rat, Canine, Chicken/Avian, Porcine, Rabbit, Zebrafish

Host

Rabbit

Isotype

IgG

Vial size

0.1 mg

Concentration

1.57 mg/ml (by UV absorbance at 280 nm)

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Western blot using Affinity Purified anti-CaM Kinase II antibody shows detection of a band ~54 kDa corresponding to human alpha CaM Kinase II (arrowhead lane 1). Specific reactivity with this band is blocked when the antibody is preincubated with the immunizing peptide (lane 2). Approximately 35 µg of a mouse brain whole cell lysate was separated by 4-20% SDS-PAGE and transferred onto nitrocellulose. CaM Kinase II was similarly detected on lysates from rat brain (not shown). After blocking the membrane was probed with the primary antibody diluted to 1:1,500 for 2h at room temperature followed by washes and reaction with a 1:10,000 dilution of IRDye(TM)800 conjugated Gta-Rabbit IgG [H&L] MX for 45 min at room temperature. IRDye(TM)800 fluorescence image was captured using the Odyssey(R) Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.