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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [7]
- Comments [0]
- Validations
- Western blot [1]
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- Product number
- NB300-184 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB300-184, RRID:AB_2068102
- Product name
- Rabbit Polyclonal CaM Kinase II alpha Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specific for the ~50k alpha-CaM Kinase II and the ~60k beta-CaM Kinase II proteins phosphorylated at Thr286. Immunolabeling is blocked by the gamma-phosphatase treatment
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references CAMKIIγ suppresses an efferocytosis pathway in macrophages and promotes atherosclerotic plaque necrosis.
Translocation of CaM kinase II to synaptic sites in vivo.
Autophosphorylated calcium/calmodulin-dependent protein kinase II alpha (CaMKII alpha) reversibly targets to and phosphorylates N-methyl-D-aspartate receptor subunit 2B (NR2B) in cerebral ischemia and reperfusion in hippocampus of rats.
Neuronal CA2+/calmodulin-dependent protein kinase II: the role of structure and autoregulation in cellular function.
Inhibitory autophosphorylation of CaMKII controls PSD association, plasticity, and learning.
Inhibitory autophosphorylation of CaMKII controls PSD association, plasticity, and learning.
Signal transduction molecules at the glutamatergic postsynaptic membrane.
Doran AC, Ozcan L, Cai B, Zheng Z, Fredman G, Rymond CC, Dorweiler B, Sluimer JC, Hsieh J, Kuriakose G, Tall AR, Tabas I
The Journal of clinical investigation 2017 Nov 1;127(11):4075-4089
The Journal of clinical investigation 2017 Nov 1;127(11):4075-4089
Translocation of CaM kinase II to synaptic sites in vivo.
Gleason MR, Higashijima S, Dallman J, Liu K, Mandel G, Fetcho JR
Nature neuroscience 2003 Mar;6(3):217-8
Nature neuroscience 2003 Mar;6(3):217-8
Autophosphorylated calcium/calmodulin-dependent protein kinase II alpha (CaMKII alpha) reversibly targets to and phosphorylates N-methyl-D-aspartate receptor subunit 2B (NR2B) in cerebral ischemia and reperfusion in hippocampus of rats.
Meng F, Guo J, Zhang Q, Song B, Zhang G
Brain research 2003 Mar 28;967(1-2):161-9
Brain research 2003 Mar 28;967(1-2):161-9
Neuronal CA2+/calmodulin-dependent protein kinase II: the role of structure and autoregulation in cellular function.
Hudmon A, Schulman H
Annual review of biochemistry 2002;71:473-510
Annual review of biochemistry 2002;71:473-510
Inhibitory autophosphorylation of CaMKII controls PSD association, plasticity, and learning.
Elgersma Y, Fedorov NB, Ikonen S, Choi ES, Elgersma M, Carvalho OM, Giese KP, Silva AJ
Neuron 2002 Oct 24;36(3):493-505
Neuron 2002 Oct 24;36(3):493-505
Inhibitory autophosphorylation of CaMKII controls PSD association, plasticity, and learning.
Elgersma Y, Fedorov NB, Ikonen S, Choi ES, Elgersma M, Carvalho OM, Giese KP, Silva AJ
Neuron 2002 Oct 24;36(3):493-505
Neuron 2002 Oct 24;36(3):493-505
Signal transduction molecules at the glutamatergic postsynaptic membrane.
Kennedy MB
Brain research. Brain research reviews 1998 May;26(2-3):243-57
Brain research. Brain research reviews 1998 May;26(2-3):243-57
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Supportive validation
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- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CaMKII alpha [p Thr286] Antibody [NB300-184] - Anaylsis of Rat brain lysate showing specific immunolabeling of the ~50k a- and the ~60k beta-CaM Kinase II phosphorylated at Thr286 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lambda-Ptase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the Anti-Thr286 CaM Kinase II. The immunolabeling is completely eliminated by treatment with lambda-Ptase.
