MA1-12028

antibody from Invitrogen Antibodies

Targeting: CD8B CD8B1

Flow cytometry (Recommended by provider)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA1-12028

Provider

Invitrogen Antibodies

Product name

CD8 Monoclonal Antibody (17D8), FITC

Antibody type

Monoclonal

Antigen

Other

Reactivity

Human

Host

Mouse

Conjugate

Green dye

Isotype

IgG

Antibody clone number

17D8

Vial size

100 Tests

Concentration

0.02 mg/mL

Storage

4° C, store in dark, DO NOT FREEZE!

References

Submitted references

The Effect of miR-520b on Macrophage Polarization and T Cell Immunity by Targeting PTEN in Breast Cancer.

Zhu Q, Yuan J, He Y, Hu Y
Journal of oncology 2021;2021:5170496

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 6 miR-520b inhibitor enhanced T cell activation in the tumor environment and guided the polarization of macrophages to M1, thereby inhibiting tumor growth. (a) The proportion of CD3, CD4, CD8, and NK1.1 cells. (b) The proportion of CD4+IFN gamma and FOXP3 cells. (c) The proportion of CD206 and CD68 cells. The proportion of CD3, CD4, CD8, NK1.1, CD4+IFN gamma , and CD68 cells increased, while FOXP3 and CD206 cells decreased in miR-520b inhibitor group compared with inhibitor NC group. However, the proportion of CD3, CD4, CD8, NK1.1, CD4+IFN gamma , and CD68 cells decreased, while FOXP3 and CD206 cells increased after the addition of si PTEN . All experiments were repeated three times; # P < 0.05 vs the inhibitor NC group, and P < 0.05 vs the miR-520b inhibitor + siNC group. Differences between the two groups were analyzed using the student's t -test. One-way analysis of variance (ANOVA) was used for comparison between multiple groups.

Conjugate

Green dye