MHCD0826

antibody from Invitrogen Antibodies

Targeting: CD8B CD8B1

Flow cytometry (Recommended by provider)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MHCD0826

Provider

Invitrogen Antibodies

Product name

CD8 Monoclonal Antibody (3B5), Alexa Fluor™ 405

Antibody type

Monoclonal

Antigen

Other

Description

For fluorochrome conjugated antibodies only, light exposure should be avoided. Use dim light during handling, incubation with cells, and prior to analysis. It is recommended that cells be analyzed within 18 hours of staining. If the reagent is being diluted, it is recommended that only the quantity to be used within one week be diluted.

Reactivity

Human

Host

Mouse

Conjugate

Blue dye

Isotype

IgG

Antibody clone number

3B5

Vial size

500 µL

Storage

4° C

References

Submitted references

Co-immunization of DNA and Protein in the Same Anatomical Sites Induces Superior Protective Immune Responses against SHIV Challenge.

Felber BK, Lu Z, Hu X, Valentin A, Rosati M, Remmel CAL, Weiner JA, Carpenter MC, Faircloth K, Stanfield-Oakley S, Williams WB, Shen X, Tomaras GD, LaBranche CC, Montefiori D, Trinh HV, Rao M, Alam MS, Vandergrift NA, Saunders KO, Wang Y, Rountree W, Das J, Alter G, Reed SG, Aye PP, Schiro F, Pahar B, Dufour JP, Veazey RS, Marx PA, Venzon DJ, Shaw GM, Ferrari G, Ackerman ME, Haynes BF, Pavlakis GN
Cell reports 2020 May 12;31(6):107624

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Gag and env conserved element CE DNA vaccines elicit broad cytotoxic T cell responses targeting subdominant epitopes of HIV and SIV Able to recognize virus-infected cells in macaques.

Hu X, Lu Z, Valentin A, Rosati M, Broderick KE, Sardesai NY, Marx PA, Mullins JI, Pavlakis GN, Felber BK
Human vaccines & immunotherapeutics 2018;14(9):2163-2177

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Triggering of TLR-3, -4, NOD2, and DC-SIGN reduces viral replication and increases T-cell activation capacity of HIV-infected human dendritic cells.

Cardinaud S, Urrutia A, Rouers A, Coulon PG, Kervevan J, Richetta C, Bet A, Maze EA, Larsen M, Iglesias MC, Appay V, Graff-Dubois S, Moris A
European journal of immunology 2017 May;47(5):818-829

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CD8(+) T Cells from Human Neonates Are Biased toward an Innate Immune Response.

Galindo-Albarrán AO, López-Portales OH, Gutiérrez-Reyna DY, Rodríguez-Jorge O, Sánchez-Villanueva JA, Ramírez-Pliego O, Bergon A, Loriod B, Holota H, Imbert J, Hernández-Mendoza A, Ferrier P, Carrillo-de Santa Pau E, Valencia A, Spicuglia S, Santana MA
Cell reports 2016 Nov 15;17(8):2151-2160

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Altered response hierarchy and increased T-cell breadth upon HIV-1 conserved element DNA vaccination in macaques.

Kulkarni V, Valentin A, Rosati M, Alicea C, Singh AK, Jalah R, Broderick KE, Sardesai NY, Le Gall S, Mothe B, Brander C, Rolland M, Mullins JI, Pavlakis GN, Felber BK
PloS one 2014;9(1):e86254

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DNA vaccination by intradermal electroporation induces long-lasting immune responses in rhesus macaques.

Kulkarni V, Rosati M, Jalah R, Ganneru B, Alicea C, Yu L, Guan Y, LaBranche C, Montefiori DC, King AD, Valentin A, Pavlakis GN, Felber BK
Journal of medical primatology 2014 Oct;43(5):329-40

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Comparative analysis of SIV-specific cellular immune responses induced by different vaccine platforms in rhesus macaques.

Valentin A, McKinnon K, Li J, Rosati M, Kulkarni V, Pilkington GR, Bear J, Alicea C, Vargas-Inchaustegui DA, Jean Patterson L, Pegu P, Liyanage NPM, Gordon SN, Vaccari M, Wang Y, Hogg AE, Frey B, Sui Y, Reed SG, Sardesai NY, Berzofsky JA, Franchini G, Robert-Guroff M, Felber BK, Pavlakis GN
Clinical immunology (Orlando, Fla.) 2014 Nov;155(1):91-107

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Syncytin proteins incorporated in placenta exosomes are important for cell uptake and show variation in abundance in serum exosomes from patients with preeclampsia.

Vargas A, Zhou S, Éthier-Chiasson M, Flipo D, Lafond J, Gilbert C, Barbeau B
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2014 Aug;28(8):3703-19

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IL-12 DNA as molecular vaccine adjuvant increases the cytotoxic T cell responses and breadth of humoral immune responses in SIV DNA vaccinated macaques.

Jalah R, Patel V, Kulkarni V, Rosati M, Alicea C, Ganneru B, von Gegerfelt A, Huang W, Guan Y, Broderick KE, Sardesai NY, LaBranche C, Montefiori DC, Pavlakis GN, Felber BK
Human vaccines & immunotherapeutics 2012 Nov 1;8(11):1620-9

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Emergence of simian immunodeficiency virus-specific cytotoxic CD4+ T cells and increased humoral responses correlate with control of rebounding viremia in CD8-depleted macaques infected with Rev-independent live-attenuated simian immunodeficiency virus.

von Gegerfelt A, Valentin A, Alicea C, Van Rompay KK, Marthas ML, Montefiori DC, Pavlakis GN, Felber BK
Journal of immunology (Baltimore, Md. : 1950) 2010 Sep 15;185(6):3348-58

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Conjugate

Blue dye

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Conjugate

Blue dye

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Conjugate

Blue dye

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Conjugate

Blue dye

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Conjugate

Blue dye

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Conjugate

Blue dye

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig 4 Magnitude and longevity of cellular responses in macaques vaccinated via the ID/EP route. Cellular immune responses were measured in PBMC upon stimulation with a mac239-specific peptide pool (15-mer overlapping by 11 AA) at start of the study (V1), 2 weeks after vaccination with gp160 (V3 wk2), gp120 (V4 wk2), and gp140 (V5 wk2). The responses are shown as % SIV Env-specific IFN-gamma + T cells among CD4 + and CD8 + (A); transitional (TM; CD28 + CD95 + CCR7 - ) and effector memory (EM; CD28 - CD95 + CCR7 - ) (B) T cells. The frequency of the Env-specific IFN-gamma + granzyme B + T cells is shown (C). The animals were monitored for ~1 year after the last vaccination, and the cellular responses were measured at V5 wk57. Asterisk marks sample not available.

Conjugate

Blue dye