Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Other assay [7]
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Validation data
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- Product number
- MHCD0826 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD8 Monoclonal Antibody (3B5), Alexa Fluor™ 405
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- For fluorochrome conjugated antibodies only, light exposure should be avoided. Use dim light during handling, incubation with cells, and prior to analysis. It is recommended that cells be analyzed within 18 hours of staining. If the reagent is being diluted, it is recommended that only the quantity to be used within one week be diluted.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Blue dye
- Isotype
- IgG
- Antibody clone number
- 3B5
- Vial size
- 500 µL
- Storage
- 4° C
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Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Conjugate
- Blue dye
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Conjugate
- Blue dye
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Conjugate
- Blue dye
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Conjugate
- Blue dye
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Conjugate
- Blue dye
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Conjugate
- Blue dye
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig 4 Magnitude and longevity of cellular responses in macaques vaccinated via the ID/EP route. Cellular immune responses were measured in PBMC upon stimulation with a mac239-specific peptide pool (15-mer overlapping by 11 AA) at start of the study (V1), 2 weeks after vaccination with gp160 (V3 wk2), gp120 (V4 wk2), and gp140 (V5 wk2). The responses are shown as % SIV Env-specific IFN-gamma + T cells among CD4 + and CD8 + (A); transitional (TM; CD28 + CD95 + CCR7 - ) and effector memory (EM; CD28 - CD95 + CCR7 - ) (B) T cells. The frequency of the Env-specific IFN-gamma + granzyme B + T cells is shown (C). The animals were monitored for ~1 year after the last vaccination, and the cellular responses were measured at V5 wk57. Asterisk marks sample not available.
- Conjugate
- Blue dye