Antibody data
- Antibody Data
- Antigen structure
- References [17]
- Comments [0]
- Validations
- Flow cytometry [1]
- Other assay [5]
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- Product number
- MHCD0801 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD8 Monoclonal Antibody (3B5), FITC
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Commonly used, FITC conjugates provide relatively high absorptivity, excellent fluorescence quantum yield, and good water solubility.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Green dye
- Isotype
- IgG
- Antibody clone number
- 3B5
- Vial size
- 500 µL
- Storage
- 4° C, store in dark
Submitted references Expansion of Group 2 Innate Lymphoid Cells in Patients with End-Stage Renal Disease and Their Clinical Significance.
Merkel cell polyomavirus-specific immune responses in patients with Merkel cell carcinoma receiving anti-PD-1 therapy.
Effects of iodine-131 radiotherapy on Th17/Tc17 and Treg/Th17 cells of patients with differentiated thyroid carcinoma.
Programmed Death 1 Expression on CD4(+) T Cells Predicts Mortality after Allogeneic Stem Cell Transplantation.
TLR and NLRP3 inflammasome-dependent innate immune responses to tumor-derived autophagosomes (DRibbles).
Redefined clinical features and diagnostic criteria in autoimmune polyendocrinopathy-candidiasis-ectodermal dystrophy.
Phase I Hepatic Immunotherapy for Metastases Study of Intra-Arterial Chimeric Antigen Receptor-Modified T-cell Therapy for CEA+ Liver Metastases.
Anti-inflammatory/regulatory cytokine microenvironment mediated by IL-4 and IL-10 coordinates the immune response in hemophilia A patients infected chronically with hepatitis C virus.
Immunological/virological peripheral blood biomarkers and distinct patterns of sleeping quality in chronic hepatitis C patients.
Low-dose rabbit antithymocyte globulin induction therapy results in prolonged selective lymphocyte depletion irrespective of maintenance immunosuppression.
In vivo inhibition of human CD19-targeted effector T cells by natural T regulatory cells in a xenotransplant murine model of B cell malignancy.
Identification of a NCR+/NKG2D+/LFA-1(low)/CD94(-) immature human NK cell subset.
Transmission of HIV-1 by primary human uterine epithelial cells and stromal fibroblasts.
Selective depletion of donor alloreactive T cells without loss of antiviral or antileukemic responses.
Expression of cutaneous lymphocyte-associated antigen by CD8(+) T cells specific for a skin-tropic virus.
Blister fluid T lymphocytes during toxic epidermal necrolysis are functional cytotoxic cells which express human natural killer (NK) inhibitory receptors.
CD3 hyporesponsiveness and in vitro apoptosis are features of T cells from both malignant and nonmalignant secondary lymphoid organs.
Liu GY, Deng XH, Li X, Cao YJ, Xing YF, Zhou P, Lei AH, Yang Q, Deng K, Zhang H, Zhou J
Journal of immunology (Baltimore, Md. : 1950) 2020 Jul 1;205(1):36-44
Journal of immunology (Baltimore, Md. : 1950) 2020 Jul 1;205(1):36-44
Merkel cell polyomavirus-specific immune responses in patients with Merkel cell carcinoma receiving anti-PD-1 therapy.
Miller NJ, Church CD, Fling SP, Kulikauskas R, Ramchurren N, Shinohara MM, Kluger HM, Bhatia S, Lundgren L, Cheever MA, Topalian SL, Nghiem P
Journal for immunotherapy of cancer 2018 Nov 27;6(1):131
Journal for immunotherapy of cancer 2018 Nov 27;6(1):131
Effects of iodine-131 radiotherapy on Th17/Tc17 and Treg/Th17 cells of patients with differentiated thyroid carcinoma.
Zhang L, Chen J, Xu C, Qi L, Ren Y
Experimental and therapeutic medicine 2018 Mar;15(3):2661-2666
Experimental and therapeutic medicine 2018 Mar;15(3):2661-2666
Programmed Death 1 Expression on CD4(+) T Cells Predicts Mortality after Allogeneic Stem Cell Transplantation.
Schade H, Sen S, Neff CP, Freed BM, Gao D, Gutman JA, Palmer BE
Biology of blood and marrow transplantation : journal of the American Society for Blood and Marrow Transplantation 2016 Dec;22(12):2172-2179
Biology of blood and marrow transplantation : journal of the American Society for Blood and Marrow Transplantation 2016 Dec;22(12):2172-2179
TLR and NLRP3 inflammasome-dependent innate immune responses to tumor-derived autophagosomes (DRibbles).
Xing Y, Cao R, Hu HM
Cell death & disease 2016 Aug 4;7(8):e2322
Cell death & disease 2016 Aug 4;7(8):e2322
Redefined clinical features and diagnostic criteria in autoimmune polyendocrinopathy-candidiasis-ectodermal dystrophy.
Ferre EM, Rose SR, Rosenzweig SD, Burbelo PD, Romito KR, Niemela JE, Rosen LB, Break TJ, Gu W, Hunsberger S, Browne SK, Hsu AP, Rampertaap S, Swamydas M, Collar AL, Kong HH, Lee CR, Chascsa D, Simcox T, Pham A, Bondici A, Natarajan M, Monsale J, Kleiner DE, Quezado M, Alevizos I, Moutsopoulos NM, Yockey L, Frein C, Soldatos A, Calvo KR, Adjemian J, Similuk MN, Lang DM, Stone KD, Uzel G, Kopp JB, Bishop RJ, Holland SM, Olivier KN, Fleisher TA, Heller T, Winer KK, Lionakis MS
JCI insight 2016 Aug 18;1(13)
JCI insight 2016 Aug 18;1(13)
Phase I Hepatic Immunotherapy for Metastases Study of Intra-Arterial Chimeric Antigen Receptor-Modified T-cell Therapy for CEA+ Liver Metastases.
Katz SC, Burga RA, McCormack E, Wang LJ, Mooring W, Point GR, Khare PD, Thorn M, Ma Q, Stainken BF, Assanah EO, Davies R, Espat NJ, Junghans RP
Clinical cancer research : an official journal of the American Association for Cancer Research 2015 Jul 15;21(14):3149-59
Clinical cancer research : an official journal of the American Association for Cancer Research 2015 Jul 15;21(14):3149-59
Anti-inflammatory/regulatory cytokine microenvironment mediated by IL-4 and IL-10 coordinates the immune response in hemophilia A patients infected chronically with hepatitis C virus.
Pimentel JP, Chaves DG, Araújo AR, de Araújo EM, da Silva Fraporti L, Neves WL, Tarragô AM, Torres KL, Gentz SH, Teixeira-Carvalho A, Martins-Filho OA, Malheiro A
Journal of medical virology 2013 Jun;85(6):1009-18
Journal of medical virology 2013 Jun;85(6):1009-18
Immunological/virological peripheral blood biomarkers and distinct patterns of sleeping quality in chronic hepatitis C patients.
de Almeida CM, de Lima TA, Castro DB, Torres KL, da Silva Braga W, Peruhype-Magalhães V, Teixeira-Carvalho A, Martins-Filho OA, Malheiro A
Scandinavian journal of immunology 2011 May;73(5):486-95
Scandinavian journal of immunology 2011 May;73(5):486-95
Low-dose rabbit antithymocyte globulin induction therapy results in prolonged selective lymphocyte depletion irrespective of maintenance immunosuppression.
Pankewycz O, Leca N, Kohli R, Wallace PK, Said M, Feng L, Alnimri M, Patel S, Laftavi MR
Transplantation proceedings 2011 Mar;43(2):462-5
Transplantation proceedings 2011 Mar;43(2):462-5
In vivo inhibition of human CD19-targeted effector T cells by natural T regulatory cells in a xenotransplant murine model of B cell malignancy.
Lee JC, Hayman E, Pegram HJ, Santos E, Heller G, Sadelain M, Brentjens R
Cancer research 2011 Apr 15;71(8):2871-81
Cancer research 2011 Apr 15;71(8):2871-81
Identification of a NCR+/NKG2D+/LFA-1(low)/CD94(-) immature human NK cell subset.
Zamai L, Galeotti L, Del Zotto G, Canonico B, Mirandola P, Papa S
Cytometry. Part A : the journal of the International Society for Analytical Cytology 2009 Nov;75(11):893-901
Cytometry. Part A : the journal of the International Society for Analytical Cytology 2009 Nov;75(11):893-901
Transmission of HIV-1 by primary human uterine epithelial cells and stromal fibroblasts.
Asin SN, Fanger MW, Wildt-Perinic D, Ware PL, Wira CR, Howell AL
The Journal of infectious diseases 2004 Jul 15;190(2):236-45
The Journal of infectious diseases 2004 Jul 15;190(2):236-45
Selective depletion of donor alloreactive T cells without loss of antiviral or antileukemic responses.
Amrolia PJ, Muccioli-Casadei G, Yvon E, Huls H, Sili U, Wieder ED, Bollard C, Michalek J, Ghetie V, Heslop HE, Molldrem JJ, Rooney CM, Schlinder J, Vitetta E, Brenner MK
Blood 2003 Sep 15;102(6):2292-9
Blood 2003 Sep 15;102(6):2292-9
Expression of cutaneous lymphocyte-associated antigen by CD8(+) T cells specific for a skin-tropic virus.
Koelle DM, Liu Z, McClurkan CM, Topp MS, Riddell SR, Pamer EG, Johnson AS, Wald A, Corey L
The Journal of clinical investigation 2002 Aug;110(4):537-48
The Journal of clinical investigation 2002 Aug;110(4):537-48
Blister fluid T lymphocytes during toxic epidermal necrolysis are functional cytotoxic cells which express human natural killer (NK) inhibitory receptors.
Le Cleach L, Delaire S, Boumsell L, Bagot M, Bourgault-Villada I, Bensussan A, Roujeau JC
Clinical and experimental immunology 2000 Jan;119(1):225-30
Clinical and experimental immunology 2000 Jan;119(1):225-30
CD3 hyporesponsiveness and in vitro apoptosis are features of T cells from both malignant and nonmalignant secondary lymphoid organs.
Agrawal S, Marquet J, Delfau-Larue MH, Copie-Bergman C, Jouault H, Reyes F, Bensussan A, Farcet JP
The Journal of clinical investigation 1998 Nov 1;102(9):1715-23
The Journal of clinical investigation 1998 Nov 1;102(9):1715-23
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human peripheral blood lymphocytes were stained using FITC of anti-human CD8 monoclonal antibody (clone 3B5). The negative control profiles represent unstained cells.
- Conjugate
- Green dye
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Conjugate
- Green dye
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Conjugate
- Green dye
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Conjugate
- Green dye
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Conjugate
- Green dye
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 7 Endocytosis was necessary in the process of DRibbles-inflammasome response. ( a ) CypHer5E-labeled DRibbles and lysotraker Green-labeled lysosome were collocated in THP-1 cells after 6 h incubation, and the nucleus were labeled with DAPI. ( b ) The endocytosis frequency of DRibbles by THP-1 cells was measured by FACS. DCs were pre-treated with ExoA (1 mu M), Cytochalasin-D (Cyto-D, 1 mu g/ml), and NH 4 Cl (20 mu M) for 30 min, then incubated with CypHer5E stained DRibbles (100 mu g/ml) overnight. ( c ) Primed THP-1 cells were pre-treated with cytochalasin-D, ExoA, and NH 4 Cl for 1 h, then stimulated with ATP/LPS (5 mM)/(10 ng/ml) and DRibbles (100 mu g/ml) overnight. The supernatant IL-1 beta was detected by ELISA. ( d ) The frequency of IFN- gamma + CD8 + , IFN- gamma + CD4 + T cells induced by pp65 DRibbles at indicated doses were measured by ICS, and ( e ) pp65 protein (10 mu g/ml) and pp65 DRibbles (10 mu g/ml). Results are shown as the means+S.D. and representative of two or more independent experiments. PBMC is from 3 donors
- Conjugate
- Green dye