Antibody data
- Antibody Data
- Antigen structure
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- Comments [0]
- Validations
- Flow cytometry [1]
- Other assay [5]
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Validation data
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- Product number
- MHCD0801-4 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD8 Monoclonal Antibody (3B5), FITC
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Commonly used, FITC conjugates provide relatively high absorptivity, excellent fluorescence quantum yield, and good water solubility.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Green dye
- Isotype
- IgG
- Antibody clone number
- 3B5
- Vial size
- 2 mL
- Storage
- 4° C, store in dark
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human peripheral blood lymphocytes were stained using FITC of anti-human CD8 monoclonal antibody (clone 3B5). The negative control profiles represent unstained cells.
- Conjugate
- Green dye
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Conjugate
- Green dye
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Conjugate
- Green dye
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Conjugate
- Green dye
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Conjugate
- Green dye
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 7 Endocytosis was necessary in the process of DRibbles-inflammasome response. ( a ) CypHer5E-labeled DRibbles and lysotraker Green-labeled lysosome were collocated in THP-1 cells after 6 h incubation, and the nucleus were labeled with DAPI. ( b ) The endocytosis frequency of DRibbles by THP-1 cells was measured by FACS. DCs were pre-treated with ExoA (1 mu M), Cytochalasin-D (Cyto-D, 1 mu g/ml), and NH 4 Cl (20 mu M) for 30 min, then incubated with CypHer5E stained DRibbles (100 mu g/ml) overnight. ( c ) Primed THP-1 cells were pre-treated with cytochalasin-D, ExoA, and NH 4 Cl for 1 h, then stimulated with ATP/LPS (5 mM)/(10 ng/ml) and DRibbles (100 mu g/ml) overnight. The supernatant IL-1 beta was detected by ELISA. ( d ) The frequency of IFN- gamma + CD8 + , IFN- gamma + CD4 + T cells induced by pp65 DRibbles at indicated doses were measured by ICS, and ( e ) pp65 protein (10 mu g/ml) and pp65 DRibbles (10 mu g/ml). Results are shown as the means+S.D. and representative of two or more independent experiments. PBMC is from 3 donors
- Conjugate
- Green dye