MHCD0804

antibody from Invitrogen Antibodies

Targeting: CD8B CD8B1

Provider product page for MHCD0804

Flow cytometry

Other assay

Antibody data

Antibody Data
Antigen structure
References [13]
Comments [0]
Validations
Flow cytometry [3]
Other assay [2]

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Product number: MHCD0804 - Provider product page
Provider: Invitrogen Antibodies
Product name: CD8 Monoclonal Antibody (3B5), PE
Antibody type: Monoclonal
Antigen: Other
Description: R-phycoerythrin (PE) is a stable and highly soluble phycobiliprotein which provides maximal absorbance and fluorescence without susceptibility to internal or external fluorescence quenching, thus providing an exceptional quantum yields and molar extinction coefficients.
Reactivity: Human
Host: Mouse
Conjugate: Yellow dye
Isotype: IgG
Antibody clone number: 3B5
Vial size: 500 µL
Storage: 4° C, store in dark

CD8B protein structure - MHCD0804 shown in red.

Submitted references Recruitment of highly cytotoxic CD8(+) T cell receptors in mild SARS-CoV-2 infection.

Wagner KI, Mateyka LM, Jarosch S, Grass V, Weber S, Schober K, Hammel M, Burrell T, Kalali B, Poppert H, Beyer H, Schambeck S, Holdenrieder S, Strötges-Achatz A, Haselmann V, Neumaier M, Erber J, Priller A, Yazici S, Roggendorf H, Odendahl M, Tonn T, Dick A, Witter K, Mijočević H, Protzer U, Knolle PA, Pichlmair A, Crowell CS, Gerhard M, D'Ippolito E, Busch DH
Cell reports 2022 Jan 11;38(2):110214

Targeted T cell receptor gene editing provides predictable T cell product function for immunotherapy.

Müller TR, Jarosch S, Hammel M, Leube J, Grassmann S, Bernard B, Effenberger M, Andrä I, Chaudhry MZ, Käuferle T, Malo A, Cicin-Sain L, Steinberger P, Feuchtinger T, Protzer U, Schumann K, Neuenhahn M, Schober K, Busch DH
Cell reports. Medicine 2021 Aug 17;2(8):100374

Interferon-α alters host glycosylation machinery during treated HIV infection.

Giron LB, Colomb F, Papasavvas E, Azzoni L, Yin X, Fair M, Anzurez A, Damra M, Mounzer K, Kostman JR, Tebas P, O'Doherty U, Tateno H, Liu Q, Betts MR, Montaner LJ, Abdel-Mohsen M
EBioMedicine 2020 Sep;59:102945

Blocking interaction between SHP2 and PD-1 denotes a novel opportunity for developing PD-1 inhibitors.

Fan Z, Tian Y, Chen Z, Liu L, Zhou Q, He J, Coleman J, Dong C, Li N, Huang J, Xu C, Zhang Z, Gao S, Zhou P, Ding K, Chen L
EMBO molecular medicine 2020 Jun 8;12(6):e11571

Simultaneous, Single-Cell Measurement of Messenger RNA, Cell Surface Proteins, and Intracellular Proteins.

Soh KT, Tario JD Jr, Colligan S, Maguire O, Pan D, Minderman H, Wallace PK
Current protocols in cytometry 2016 Jan 6;75:7.45.1-7.45.33

IL-12-secreting CD19-targeted cord blood-derived T cells for the immunotherapy of B-cell acute lymphoblastic leukemia.

Pegram HJ, Purdon TJ, van Leeuwen DG, Curran KJ, Giralt SA, Barker JN, Brentjens RJ
Leukemia 2015 Feb;29(2):415-22

Cross-presentation of viral antigens in dribbles leads to efficient activation of virus-specific human memory T cells.

Ye W, Xing Y, Paustian C, van de Ven R, Moudgil T, Hilton TL, Fox BA, Urba WJ, Zhao W, Hu HM
Journal of translational medicine 2014 Apr 16;12:100

Cancer regression and neurological toxicity following anti-MAGE-A3 TCR gene therapy.

Morgan RA, Chinnasamy N, Abate-Daga D, Gros A, Robbins PF, Zheng Z, Dudley ME, Feldman SA, Yang JC, Sherry RM, Phan GQ, Hughes MS, Kammula US, Miller AD, Hessman CJ, Stewart AA, Restifo NP, Quezado MM, Alimchandani M, Rosenberg AZ, Nath A, Wang T, Bielekova B, Wuest SC, Akula N, McMahon FJ, Wilde S, Mosetter B, Schendel DJ, Laurencot CM, Rosenberg SA
Journal of immunotherapy (Hagerstown, Md. : 1997) 2013 Feb;36(2):133-51

Identification of a NCR+/NKG2D+/LFA-1(low)/CD94(-) immature human NK cell subset.

Zamai L, Galeotti L, Del Zotto G, Canonico B, Mirandola P, Papa S
Cytometry. Part A : the journal of the International Society for Analytical Cytology 2009 Nov;75(11):893-901

Flow cytometry in the bone marrow staging of mature B-cell neoplasms.

Stacchini A, Demurtas A, Godio L, Martini G, Antinoro V, Palestro G
Cytometry. Part B, Clinical cytometry 2003 Jul;54(1):10-8

Regulation of thymic epithelium by keratinocyte growth factor.

Erickson M, Morkowski S, Lehar S, Gillard G, Beers C, Dooley J, Rubin JS, Rudensky A, Farr AG
Blood 2002 Nov 1;100(9):3269-78

Reactive oxygen intermediates during programmed cell death induced in the thymus of the Ts(1716)65Dn mouse, a murine model for human Down's syndrome.

Paz-Miguel JE, Flores R, Sánchez-Velasco P, Ocejo-Vinyals G, Escribano de Diego J, López de Rego J, Leyva-Cobián F
Journal of immunology (Baltimore, Md. : 1950) 1999 Nov 15;163(10):5399-410

Gammadelta+ T cells preferentially respond to live rather than killed malaria parasites.

Waterfall M, Black A, Riley E
Infection and immunity 1998 May;66(5):2393-8

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Dual color flow cytometry dot plot showing mouse anti human CD8 RPE vs. mouse anti human CD4 biotin followed by streptavidin RPE-Alexa Fluor® 610. Human mononuclear cells were stained with mouse anti human CD4 biotin (Product # MHCD0415), washed, stained with streptavidin RPE-Alexa Fluor® 610 (Product # S-20982) and mouse anti human CD8 RPE (Product # MHCD0804), washed, and analyzed on a flow cytometer with a 488 nm excitation source.
Conjugate: Yellow dye

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: An antibody complex was made by using the Zenon® Alexa Fluor® 680-R-Phycoerythrin Mouse IgG1 Labeling Kit (Product # Z-25022) and mouse anti human CD4. Human mononuclear cells were stained with mouse anti human CD8 RPE (Product # MHCD0804) and the labled anti-CD4 antibody complex and analyzed by flow cytometry using a 488 nm Laser.
Conjugate: Yellow dye

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Human peripheral blood lymphocytes were stained using R-PE of anti-human CD8 monoclonal antibody (clone 3B5). The negative control profiles represent unstained cells.
Conjugate: Yellow dye

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL
Conjugate: Yellow dye

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 4 Antigen-specific CD8 + and CD4 + T cells response following stimulation by DRibbles or cell lysates. (A) The ubiquitinated proteins and pp65 proteins contained in the cell lysate and DRibbles were examined by western blot. Lysates and DRibbles extracted from cell lines treated with bortezomib contained more ubiquitinated proteins than those from non-treated cells (left). The pp65 protein was detected in the lysates and DRibbles extracted from the UbiLT3 pp65 cell line. The pp65 DRibbles collected from the bortezomib-treated UbiLT3 pp65 cell line contained more 148Kd pp65 protein compared with the other groups (right). Data are representative of 3 independent experiments. (B,C) PBMCs were stimulated by DRibbles and cell lysates at the following doses: 3ug/ml, 10ug/ml and 25ug/ml. The percentage of IFN-gamma + CD8 + (B) and IFN-gamma + CD4 + cells (C) were calculated by flow cytometry. Percentages of IFN-gamma + T cells are shown as mean +- SEM. Data are representative of results from 3 independent experiments. (D) Dot plots at the antigen dose of 25ug/ml.
Conjugate: Yellow dye