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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [14]
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- Validations
- Flow cytometry [1]
- Other assay [1]
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- Product number
- MHCD0805 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD8 Monoclonal Antibody (3B5), APC
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Allophycocyanin (APC) is a stable and highly soluble phycobiliprotein that provides maximal absorbance and fluorescence without susceptibility to internal or external fluorescence quenching, thus providing exceptional quantum yields and molar extinction coefficients.
- Antibody clone number
- 3B5
Submitted references Biochemical and functional characterization of mutant KRAS epitopes validates this oncoprotein for immunological targeting.
High-Throughput Generation of Bipod (Fab × scFv) Bispecific Antibodies Exploits Differential Chain Expression and Affinity Capture.
Human CD4(+) T Cells Specific for Merkel Cell Polyomavirus Localize to Merkel Cell Carcinomas and Target a Required Oncogenic Domain.
Immunological Differences between Lymphocytic and Collagenous Colitis.
Regional Specialisation of T Cell Subsets and Apoptosis in the Human Gut Mucosa: Differences Between Ileum and Colon in Healthy Intestine and Inflammatory Bowel Diseases.
Differential Immune Profiles in Two Pandemic Influenza A(H1N1)pdm09 Virus Waves at Pandemic Epicenter.
IL-12-secreting CD19-targeted cord blood-derived T cells for the immunotherapy of B-cell acute lymphoblastic leukemia.
T cell interleukin-15 surface expression in chimpanzees infected with human immunodeficiency virus.
Human neonatal naive CD4+ T cells have enhanced activation-dependent signaling regulated by the microRNA miR-181a.
CD8+ lymphocytes do not mediate protection against acute superinfection 20 days after vaccination with a live attenuated simian immunodeficiency virus.
Phenotypic and molecular characterization of CD103+ CD4+ T cells in bronchoalveolar lavage from patients with interstitial lung diseases.
Reduced cell surface expression of CCR5 in CCR5Delta 32 heterozygotes is mediated by gene dosage, rather than by receptor sequestration.
A membrane-proximal basic domain and cysteine cluster in the C-terminal tail of CCR5 constitute a bipartite motif critical for cell surface expression.
Quantification of CD4, CCR5, and CXCR4 levels on lymphocyte subsets, dendritic cells, and differentially conditioned monocyte-derived macrophages.
Bear AS, Blanchard T, Cesare J, Ford MJ, Richman LP, Xu C, Baroja ML, McCuaig S, Costeas C, Gabunia K, Scholler J, Posey AD Jr, O'Hara MH, Smole A, Powell DJ Jr, Garcia BA, Vonderheide RH, Linette GP, Carreno BM
Nature communications 2021 Jul 16;12(1):4365
Nature communications 2021 Jul 16;12(1):4365
High-Throughput Generation of Bipod (Fab × scFv) Bispecific Antibodies Exploits Differential Chain Expression and Affinity Capture.
Nesspor TC, Kinealy K, Mazzanti N, Diem MD, Boye K, Hoffman H, Springer C, Sprenkle J, Powers G, Jiang H, La Porte SL, Ganesan R, Singh S, Zwolak A
Scientific reports 2020 May 5;10(1):7557
Scientific reports 2020 May 5;10(1):7557
Human CD4(+) T Cells Specific for Merkel Cell Polyomavirus Localize to Merkel Cell Carcinomas and Target a Required Oncogenic Domain.
Longino NV, Yang J, Iyer JG, Ibrani D, Chow IT, Laing KJ, Campbell VL, Paulson KG, Kulikauskas RM, Church CD, James EA, Nghiem P, Kwok WW, Koelle DM
Cancer immunology research 2019 Oct;7(10):1727-1739
Cancer immunology research 2019 Oct;7(10):1727-1739
Immunological Differences between Lymphocytic and Collagenous Colitis.
Carrasco A, Esteve M, Salas A, Pedrosa E, Rosinach M, Aceituno M, Zabana Y, Fernández-Bañares F
Journal of Crohn's & colitis 2016 Sep;10(9):1055-66
Journal of Crohn's & colitis 2016 Sep;10(9):1055-66
Regional Specialisation of T Cell Subsets and Apoptosis in the Human Gut Mucosa: Differences Between Ileum and Colon in Healthy Intestine and Inflammatory Bowel Diseases.
Carrasco A, Fernández-Bañares F, Pedrosa E, Salas A, Loras C, Rosinach M, Aceituno M, Andújar X, Forné M, Zabana Y, Esteve M
Journal of Crohn's & colitis 2016 Sep;10(9):1042-54
Journal of Crohn's & colitis 2016 Sep;10(9):1042-54
Differential Immune Profiles in Two Pandemic Influenza A(H1N1)pdm09 Virus Waves at Pandemic Epicenter.
Arriaga-Pizano L, Ferat-Osorio E, Rodríguez-Abrego G, Mancilla-Herrera I, Domínguez-Cerezo E, Valero-Pacheco N, Pérez-Toledo M, Lozano-Patiño F, Laredo-Sánchez F, Malagón-Rangel J, Nellen-Hummel H, González-Bonilla C, Arteaga-Troncoso G, Cérbulo-Vázquez A, Pastelin-Palacios R, Klenerman P, Isibasi A, López-Macías C
Archives of medical research 2015 Nov;46(8):651-8
Archives of medical research 2015 Nov;46(8):651-8
IL-12-secreting CD19-targeted cord blood-derived T cells for the immunotherapy of B-cell acute lymphoblastic leukemia.
Pegram HJ, Purdon TJ, van Leeuwen DG, Curran KJ, Giralt SA, Barker JN, Brentjens RJ
Leukemia 2015 Feb;29(2):415-22
Leukemia 2015 Feb;29(2):415-22
T cell interleukin-15 surface expression in chimpanzees infected with human immunodeficiency virus.
Rodriguez AR, Hodara V, Murthy K, Morrow L, Sanchez M, Bienvenu AE, Murthy KK
Cellular immunology 2014 Mar-Apr;288(1-2):24-30
Cellular immunology 2014 Mar-Apr;288(1-2):24-30
Human neonatal naive CD4+ T cells have enhanced activation-dependent signaling regulated by the microRNA miR-181a.
Palin AC, Ramachandran V, Acharya S, Lewis DB
Journal of immunology (Baltimore, Md. : 1950) 2013 Mar 15;190(6):2682-91
Journal of immunology (Baltimore, Md. : 1950) 2013 Mar 15;190(6):2682-91
CD8+ lymphocytes do not mediate protection against acute superinfection 20 days after vaccination with a live attenuated simian immunodeficiency virus.
Stebbings R, Berry N, Waldmann H, Bird P, Hale G, Stott J, North D, Hull R, Hall J, Lines J, Brown S, D'Arcy N, Davis L, Elsley W, Edwards C, Ferguson D, Allen J, Almond N
Journal of virology 2005 Oct;79(19):12264-72
Journal of virology 2005 Oct;79(19):12264-72
Phenotypic and molecular characterization of CD103+ CD4+ T cells in bronchoalveolar lavage from patients with interstitial lung diseases.
Braun RK, Foerster M, Grahmann PR, Haefner D, Workalemahu G, Kroegel C
Cytometry. Part B, Clinical cytometry 2003 Jul;54(1):19-27
Cytometry. Part B, Clinical cytometry 2003 Jul;54(1):19-27
Reduced cell surface expression of CCR5 in CCR5Delta 32 heterozygotes is mediated by gene dosage, rather than by receptor sequestration.
Venkatesan S, Petrovic A, Van Ryk DI, Locati M, Weissman D, Murphy PM
The Journal of biological chemistry 2002 Jan 18;277(3):2287-301
The Journal of biological chemistry 2002 Jan 18;277(3):2287-301
A membrane-proximal basic domain and cysteine cluster in the C-terminal tail of CCR5 constitute a bipartite motif critical for cell surface expression.
Venkatesan S, Petrovic A, Locati M, Kim YO, Weissman D, Murphy PM
The Journal of biological chemistry 2001 Oct 26;276(43):40133-45
The Journal of biological chemistry 2001 Oct 26;276(43):40133-45
Quantification of CD4, CCR5, and CXCR4 levels on lymphocyte subsets, dendritic cells, and differentially conditioned monocyte-derived macrophages.
Lee B, Sharron M, Montaner LJ, Weissman D, Doms RW
Proceedings of the National Academy of Sciences of the United States of America 1999 Apr 27;96(9):5215-20
Proceedings of the National Academy of Sciences of the United States of America 1999 Apr 27;96(9):5215-20
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human peripheral blood lymphocytes were stained using APC of anti-human CD8 monoclonal antibody (clone 3B5). The negative control profiles represent unstained cells.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 5 Functional comparison of a ""bipod"" BsAb produced using either large-scale ( A ) or high-throughput ( B ) methods. ( A ) Graph of the % CARNAVAL cell lysis vs concentration of antibody overlaid with the relative T cell activation, based on CD25 expression. The BsAb displays an EC 50 for CARNAVAL cell killing of 32 pM. ( B ) The same experiment in ( A ) was performed using the BsAb generated in small scale using high-throughput methods, and it shows the antibody has similar activity, displaying an EC 50 for CARNAVAL cell killing of 23 pM.
