PAB3088

antibody from Abnova Corporation

Targeting: CD8B CD8B1

Western blot (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB3088

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB3088, RRID:AB_1572370

Product name

CD8B polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against synthetic peptide of CD8B.

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Recent duplication of the two human CD8 beta-chain genes.

Nakayama K, Kawachi Y, Tokito S, Minami N, Yamamoto R, Imai T, Gachelin G, Nakauchi H
Journal of immunology (Baltimore, Md. : 1950) 1992 Mar 15;148(6):1919-27

---

Differential expression and regulation of the human CD8 alpha and CD8 beta chains.

Terry LA, DiSanto JP, Small TN, Flomenberg N
Tissue antigens 1990 Feb;35(2):82-91

---

Molecular biology and function of CD4 and CD8.

Parnes JR
Advances in immunology 1989;44:265-311

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of CD8B polyclonal antibody (Cat # PAB3088) in K-562 cell line lysates (35 ug/lane). CD8B (arrow) was detected using the purified polyclonal antibody.

Flow cytometry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Flow cytometric analysis of Jurkat cells using CD8B polyclonal antibody (Cat # PAB3088)(bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Validation comment

Flow Cytometry