Antibody data

Product number
12-0088-42 - Provider product page
Provider
Invitrogen Antibodies
Product name
CD8a Monoclonal Antibody (RPA-T8), PE, eBioscience™
Antibody type
Monoclonal
Antigen
Other
Description
Description: The RPA-T8 monoclonal antibody reacts with the human CD8a molecule, an approximately 32-34 kDa cell surface receptor expressed either as a heterodimer with the CD8 beta chain (CD8 alpha/beta) or as a homodimer (CD8 alpha/alpha). A majority of thymocytes and a subpopulation of mature T cells and NK cells express CD8a. CD8 binds to MHC class I and through its association with protein tyrosine kinase p56lck plays a role in T-cell development and activation of mature T cells. Applications Reported:The RPA-T8 antibody been reported for use in flow cytometric analysis. Applications Tested: Has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at test size: 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. The pre-titrated test size contains BSA and/or gelatin for protein stabilization. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 µm post-manufacturing filtered.
Reactivity
Human
Host
Mouse
Conjugate
Yellow dye
Isotype
IgG
Antibody clone number
RPA-T8
Vial size
100 Tests
Concentration
5 µL/Test
Storage
4°C, store in dark, DO NOT FREEZE!
CD8B protein structure - 12-0088-42 shown in red.