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Immunohistochemistry
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [28]
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- Validations
- Immunohistochemistry [2]
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- Product number
- MA5-16345 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD8 Monoclonal Antibody (SP16)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is tonsil tissue.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- SP16
- Vial size
- 500 µL
- Concentration
- 0.022 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Expression of programmed cell death ligand 1 (PD-L1) in advanced stage EBV-associated extranodal NK/T cell lymphoma is associated with better prognosis.
Nontuberculous mycobacterial infection with concurrent IgG4-related lymphadenopathy.
Prognostic Implication of M2 Macrophages Are Determined by the Proportional Balance of Tumor Associated Macrophages and Tumor Infiltrating Lymphocytes in Microsatellite-Unstable Gastric Carcinoma.
Tumor-Infiltrating Immune Cells Are Associated With Prognosis of Gastric Cancer.
CUX1 modulates polarization of tumor-associated macrophages by antagonizing NF-κB signaling.
High infiltration of tumor-associated macrophages in triple-negative breast cancer is associated with a higher risk of distant metastasis.
Low intraprostatic DHT promotes the infiltration of CD8+ T cells in BPH tissues via modulation of CCL5 secretion.
Prognostic implications of tumor-infiltrating FoxP3+ regulatory T cells and CD8+ cytotoxic T cells in microsatellite-unstable gastric cancers.
Expression of CD8 is associated with non-common type morphology and outcome in pediatric anaplastic lymphoma kinase-positive anaplastic large cell lymphoma.
Tumor-infiltrating lymphocytes, tumor characteristics, and recurrence in patients with early breast cancer.
T cell-activation in neuromyelitis optica lesions plays a role in their formation.
Lymphoid tissue damage in HIV-1 infection depletes naïve T cells and limits T cell reconstitution after antiretroviral therapy.
Immune response to Mycobacterium tuberculosis infection in the parietal pleura of patients with tuberculous pleurisy.
Intratumoral Immune Responses Can Distinguish New Primary and True Recurrence Types of Ipsilateral Breast Tumor Recurrences (IBTR).
Tumor-infiltrating lymphocytes predict response to anthracycline-based chemotherapy in estrogen receptor-negative breast cancer.
Anaplastic large cell lymphoma with paraneoplastic leukocytosis: a clinicopathological analysis of five cases.
Hepatitis C virus induces the expression of CCL17 and CCL22 chemokines that attract regulatory T cells to the site of infection.
CD8⁺ T cells with an intraepithelial phenotype upregulate cytotoxic function upon influenza infection in human lung.
Study of cellular immune response against Hepatitis E virus (HEV).
Spatial differences in the presence of FOXP3+ and GranzymeB+ T cells between the intra- and extravascular compartments in renal allograft vasculopathy.
Primary central nervous system peripheral T-cell lymphoma in a child.
Antagonism of TIM-1 blocks the development of disease in a humanized mouse model of allergic asthma.
Increased T-bet+ cytotoxic effectors and type I interferon-mediated processes in chronic graft-versus-host disease of the oral mucosa.
Tumor-infiltrating T cells correlate with NY-ESO-1-specific autoantibodies in ovarian cancer.
GMP-17-positive T-lymphocytes in renal tubules predict progression in early stages of IgA nephropathy.
Selective expansion of influenza A virus-specific T cells in symptomatic human carotid artery atherosclerotic plaques.
Effect of melatonin and zinc on the immune response in experimental Toxoplasma retinochoroiditis.
Intrahepatic virus-specific IL-10-producing CD8 T cells prevent liver damage during chronic hepatitis C virus infection.
Kim WY, Jung HY, Nam SJ, Kim TM, Heo DS, Kim CW, Jeon YK
Virchows Archiv : an international journal of pathology 2016 Nov;469(5):581-590
Virchows Archiv : an international journal of pathology 2016 Nov;469(5):581-590
Nontuberculous mycobacterial infection with concurrent IgG4-related lymphadenopathy.
Liu TT, Weng SW, Wang MC, Huang WT
APMIS : acta pathologica, microbiologica, et immunologica Scandinavica 2016 Mar;124(3):216-20
APMIS : acta pathologica, microbiologica, et immunologica Scandinavica 2016 Mar;124(3):216-20
Prognostic Implication of M2 Macrophages Are Determined by the Proportional Balance of Tumor Associated Macrophages and Tumor Infiltrating Lymphocytes in Microsatellite-Unstable Gastric Carcinoma.
Kim KJ, Wen XY, Yang HK, Kim WH, Kang GH
PloS one 2015;10(12):e0144192
PloS one 2015;10(12):e0144192
Tumor-Infiltrating Immune Cells Are Associated With Prognosis of Gastric Cancer.
Liu K, Yang K, Wu B, Chen H, Chen X, Chen X, Jiang L, Ye F, He D, Lu Z, Xue L, Zhang W, Li Q, Zhou Z, Mo X, Hu J
Medicine 2015 Sep;94(39):e1631
Medicine 2015 Sep;94(39):e1631
CUX1 modulates polarization of tumor-associated macrophages by antagonizing NF-κB signaling.
Kühnemuth B, Mühlberg L, Schipper M, Griesmann H, Neesse A, Milosevic N, Wissniowski T, Buchholz M, Gress TM, Michl P
Oncogene 2015 Jan 8;34(2):177-87
Oncogene 2015 Jan 8;34(2):177-87
High infiltration of tumor-associated macrophages in triple-negative breast cancer is associated with a higher risk of distant metastasis.
Yuan ZY, Luo RZ, Peng RJ, Wang SS, Xue C
OncoTargets and therapy 2014;7:1475-80
OncoTargets and therapy 2014;7:1475-80
Low intraprostatic DHT promotes the infiltration of CD8+ T cells in BPH tissues via modulation of CCL5 secretion.
Fan Y, Hu S, Liu J, Xiao F, Li X, Yu W, Cui Y, Sun M, Lv T, He Q, Jin J
Mediators of inflammation 2014;2014:397815
Mediators of inflammation 2014;2014:397815
Prognostic implications of tumor-infiltrating FoxP3+ regulatory T cells and CD8+ cytotoxic T cells in microsatellite-unstable gastric cancers.
Kim KJ, Lee KS, Cho HJ, Kim YH, Yang HK, Kim WH, Kang GH
Human pathology 2014 Feb;45(2):285-93
Human pathology 2014 Feb;45(2):285-93
Expression of CD8 is associated with non-common type morphology and outcome in pediatric anaplastic lymphoma kinase-positive anaplastic large cell lymphoma.
Abramov D, Oschlies I, Zimmermann M, Konovalov D, Damm-Welk C, Wössmann W, Klapper W
Haematologica 2013 Oct;98(10):1547-53
Haematologica 2013 Oct;98(10):1547-53
Tumor-infiltrating lymphocytes, tumor characteristics, and recurrence in patients with early breast cancer.
Kim ST, Jeong H, Woo OH, Seo JH, Kim A, Lee ES, Shin SW, Kim YH, Kim JS, Park KH
American journal of clinical oncology 2013 Jun;36(3):224-31
American journal of clinical oncology 2013 Jun;36(3):224-31
T cell-activation in neuromyelitis optica lesions plays a role in their formation.
Pohl M, Kawakami N, Kitic M, Bauer J, Martins R, Fischer MT, Machado-Santos J, Mader S, Ellwart JW, Misu T, Fujihara K, Wekerle H, Reindl M, Lassmann H, Bradl M
Acta neuropathologica communications 2013 Dec 24;1:85
Acta neuropathologica communications 2013 Dec 24;1:85
Lymphoid tissue damage in HIV-1 infection depletes naïve T cells and limits T cell reconstitution after antiretroviral therapy.
Zeng M, Southern PJ, Reilly CS, Beilman GJ, Chipman JG, Schacker TW, Haase AT
PLoS pathogens 2012 Jan;8(1):e1002437
PLoS pathogens 2012 Jan;8(1):e1002437
Immune response to Mycobacterium tuberculosis infection in the parietal pleura of patients with tuberculous pleurisy.
Caramori G, Lasagna L, Casalini AG, Adcock IM, Casolari P, Contoli M, Tafuro F, Padovani A, Chung KF, Barnes PJ, Papi A, Rindi G, Bertorelli G
PloS one 2011;6(7):e22637
PloS one 2011;6(7):e22637
Intratumoral Immune Responses Can Distinguish New Primary and True Recurrence Types of Ipsilateral Breast Tumor Recurrences (IBTR).
West NR, Panet-Raymond V, Truong PT, Alexander C, Babinszky S, Milne K, Ross LA, Loken S, Watson PH
Breast cancer : basic and clinical research 2011;5:105-15
Breast cancer : basic and clinical research 2011;5:105-15
Tumor-infiltrating lymphocytes predict response to anthracycline-based chemotherapy in estrogen receptor-negative breast cancer.
West NR, Milne K, Truong PT, Macpherson N, Nelson BH, Watson PH
Breast cancer research : BCR 2011;13(6):R126
Breast cancer research : BCR 2011;13(6):R126
Anaplastic large cell lymphoma with paraneoplastic leukocytosis: a clinicopathological analysis of five cases.
Chang IW, Chen HK, Ma MC, Huang WT
APMIS : acta pathologica, microbiologica, et immunologica Scandinavica 2011 Nov;119(11):794-801
APMIS : acta pathologica, microbiologica, et immunologica Scandinavica 2011 Nov;119(11):794-801
Hepatitis C virus induces the expression of CCL17 and CCL22 chemokines that attract regulatory T cells to the site of infection.
Riezu-Boj JI, Larrea E, Aldabe R, Guembe L, Casares N, Galeano E, Echeverria I, Sarobe P, Herrero I, Sangro B, Prieto J, Lasarte JJ
Journal of hepatology 2011 Mar;54(3):422-31
Journal of hepatology 2011 Mar;54(3):422-31
CD8⁺ T cells with an intraepithelial phenotype upregulate cytotoxic function upon influenza infection in human lung.
Piet B, de Bree GJ, Smids-Dierdorp BS, van der Loos CM, Remmerswaal EB, von der Thüsen JH, van Haarst JM, Eerenberg JP, ten Brinke A, van der Bij W, Timens W, van Lier RA, Jonkers RE
The Journal of clinical investigation 2011 Jun;121(6):2254-63
The Journal of clinical investigation 2011 Jun;121(6):2254-63
Study of cellular immune response against Hepatitis E virus (HEV).
Prabhu SB, Gupta P, Durgapal H, Rath S, Gupta SD, Acharya SK, Panda SK
Journal of viral hepatitis 2011 Aug;18(8):587-94
Journal of viral hepatitis 2011 Aug;18(8):587-94
Spatial differences in the presence of FOXP3+ and GranzymeB+ T cells between the intra- and extravascular compartments in renal allograft vasculopathy.
de Boer OJ, Teeling P, Jansen M, Ploegmakers H, van der Loos CM, Kummer JA, Florquin S, van der Wal AC
PloS one 2011 Apr 6;6(4):e18656
PloS one 2011 Apr 6;6(4):e18656
Primary central nervous system peripheral T-cell lymphoma in a child.
Gualco G, Wludarski S, Hayashi-Silva L, Medeiros Filho P, Veras G, Bacchi CE
Fetal and pediatric pathology 2010;29(4):224-30
Fetal and pediatric pathology 2010;29(4):224-30
Antagonism of TIM-1 blocks the development of disease in a humanized mouse model of allergic asthma.
Sonar SS, Hsu YM, Conrad ML, Majeau GR, Kilic A, Garber E, Gao Y, Nwankwo C, Willer G, Dudda JC, Kim H, Bailly V, Pagenstecher A, Rennert PD, Renz H
The Journal of clinical investigation 2010 Aug;120(8):2767-81
The Journal of clinical investigation 2010 Aug;120(8):2767-81
Increased T-bet+ cytotoxic effectors and type I interferon-mediated processes in chronic graft-versus-host disease of the oral mucosa.
Imanguli MM, Swaim WD, League SC, Gress RE, Pavletic SZ, Hakim FT
Blood 2009 Apr 9;113(15):3620-30
Blood 2009 Apr 9;113(15):3620-30
Tumor-infiltrating T cells correlate with NY-ESO-1-specific autoantibodies in ovarian cancer.
Milne K, Barnes RO, Girardin A, Mawer MA, Nesslinger NJ, Ng A, Nielsen JS, Sahota R, Tran E, Webb JR, Wong MQ, Wick DA, Wray A, McMurtrie E, Köbel M, Kalloger SE, Gilks CB, Watson PH, Nelson BH
PloS one 2008;3(10):e3409
PloS one 2008;3(10):e3409
GMP-17-positive T-lymphocytes in renal tubules predict progression in early stages of IgA nephropathy.
van Es LA, de Heer E, Vleming LJ, van der Wal A, Mallat M, Bajema I, Bruijn JA, de Fijter JW
Kidney international 2008 Jun;73(12):1426-33
Kidney international 2008 Jun;73(12):1426-33
Selective expansion of influenza A virus-specific T cells in symptomatic human carotid artery atherosclerotic plaques.
Keller TT, van der Meer JJ, Teeling P, van der Sluijs K, Idu MM, Rimmelzwaan GF, Levi M, van der Wal AC, de Boer OJ
Stroke 2008 Jan;39(1):174-9
Stroke 2008 Jan;39(1):174-9
Effect of melatonin and zinc on the immune response in experimental Toxoplasma retinochoroiditis.
Avunduk AM, Avunduk MC, Baltaci AK, Moğulkoç R
Ophthalmologica. Journal international d'ophtalmologie. International journal of ophthalmology. Zeitschrift fur Augenheilkunde 2007;221(6):421-5
Ophthalmologica. Journal international d'ophtalmologie. International journal of ophthalmology. Zeitschrift fur Augenheilkunde 2007;221(6):421-5
Intrahepatic virus-specific IL-10-producing CD8 T cells prevent liver damage during chronic hepatitis C virus infection.
Abel M, Sène D, Pol S, Bourlière M, Poynard T, Charlotte F, Cacoub P, Caillat-Zucman S
Hepatology (Baltimore, Md.) 2006 Dec;44(6):1607-16
Hepatology (Baltimore, Md.) 2006 Dec;44(6):1607-16
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of CD8 using anti-CD8 Monoclonal Antibody (Product # MA5-16345) in Tonsil Tissue. The recommened dilution for this antibody in immunohistochemistry applications is 1:100.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of CD8 using a monoclonal antibody (Product # MA1-39566).
