Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
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Validation data
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- Product number
- PA1-28295 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-Cdc42 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- PA1-28295 detects CDC42 from human, mouse, rat samples.
- Reactivity
- Human, Mouse, Rat
- Host
- Chicken/Avian
- Isotype
- IgY
- Vial size
- 50 µg
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Cdc42 in E coli-derived fusion protein using a Cdc42 polyclonal antibody (Product # PA1-28295) at a dilution of 1:2000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- KD of Cdc42 was achieved by transfecting A-431 with Cdc42 specific siRNAs (Silencer® select Product # s2765, s2766). Western blot analysis (Fig. a) was performed using whole cell extracts from the Cdc42 KD cells (Lane 3), non-specific scrambled siRNA transfected cells (Lane 2) and untransfected cells (Lane 1). The blot was probed with Cdc42 Polyclonal Antibody (Product # PA1-28295, 1:1000 dilution) and Goat anti-Chicken IgY (H+L) Secondary Antibody, HRP (Product # A16054, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to Cdc42. An uncharacterized band at ~80 kDa was observed in the samples tested..
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-Cdc42 Polyclonal Antibody (Product # PA1-28295) and 21 kDa band corresponding to Cdc42 was observed across cell lines and tissues tested along with an uncharacterized band at ~80 kDa. Whole cell extracts (30 µg lysate) of U-937 (Lane 1), A-431 (Lane 2), T-47D (Lane 3), K-562 (Lane 4), HeLa (Lane 5), Hep G2 (Lane 6), tissue extracts of Mouse Brain (Lane 7) and Rat Brain (Lane 8) were electrophoresed using NuPAGE™ 12% Bis-Tris Protein Gel (Product # NP0342BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence Goat anti-Chicken IgY (H+L) Secondary Antibody, HRP (Product # A16054, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).