AP7725a

antibody from Abgent

Targeting: YES1 c-yes, HsT441, Yes

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP7725a

Provider

Abgent

Proper citation

Abgent Cat#AP7725a, RRID:AB_353773

Product name

YES1 Antibody (N-term)

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Purified Rabbit Polyclonal Antibody (Pab)

Reactivity

Human, Mouse

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Antibody clone number

RB3091

Vial size

400 µl

Storage

Store at 2 to 8°C.Antibody is stable for 40 months.

Western blot

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

Western blot analysis of anti-YES Pab (Cat. #AP7725a) in mouse kidney tissue lysate. YES (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.

Primary Ab dilution

1:1000

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

YES Antibody (K11) (Cat. #AP7725a) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the YES antibody detected the YES protein (arrow).

Primary Ab dilution

1:1000

Immunohistochemistry

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

"Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma."

Primary Ab dilution

1:50~100