H00007525-M05

antibody from Abnova Corporation

Targeting: YES1 c-yes, HsT441, Yes

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

H00007525-M05

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00007525-M05, RRID:AB_894058

Product name

YES1 monoclonal antibody (M05), clone 2A12

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full length recombinant YES1.

Antigen sequence

MGCIKSKENKSPAIKYRPENTPEPVSTSVSHYGAE
PTTVSPCPSSSAKGTAVNFSSLSMTPFGGSSGVTP
FGGASSSFSVVPSSYPAGLTGGVTIFVALYDYEAR
TTEDLSFKKGERFQIINNTEGDWWEARSIATGKNG
YIPSNYVAPADSIQAEEWYFGKMGRKDAERLLLNP
GNQRGIFLVRESETTKGAYSLSIRDWDEIRGDNVK
HYKIRKLDNGGYYITTRAQFDTLQKLVKHYTEHAD
GLCHKLTTVCPTVKPQTQGLAKDAWEIPRESLRLE
VKLGQGCFGEVWMGTWNGTTKVAIKTLKPGTMMPE
AFLQEAQIMKKLRHDKLVPLYAVVSEEPIYIVTEF
MSKGSLLDFLKEGDGKYLKLPQLVDMAAQIADGMA
YIERMNYIHRDLRAANILVGENLVCKIADFGLARL
IEDNEYTARQGAKFPIKWTAPEAALYGRFTIKSDV
WSFGILQTELVTKGRVPYPGMVNREVLEQVERGYR
MPCPQGCPESLHELMNLCWKKDPDERPTFEYIQSF
LEDYFTATEPQYQPGENL


Isotype

IgG

Antibody clone number

2A12

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

YES1 monoclonal antibody (M05), clone 2A12 Western Blot analysis of YES1 expression in A-431 ( Cat # L015V1 ).

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged YES1 is approximately 10ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol