PA5-27228

antibody from Invitrogen Antibodies

Targeting: YES1 c-yes, HsT441, Yes

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-27228

Provider

Invitrogen Antibodies

Product name

YES1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: A431, Neuro 2A, C8D30, NIH-3T3, Raw264.7, PC-12, Rat2.

Concentration

1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of c-Yes using 30 µg of NIH-3T3 lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a c-Yes polyclonal antibody (Product # PA5-27228) at a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of YES1 was performed by separating 30 µg of various whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a YES1 Polyclonal Antibody (Product # PA5-27228) at a dilution of 1:500.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of YES1 was performed by separating 30 µg of various whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a YES1 Polyclonal Antibody (Product # PA5-27228) at a dilution of 1:500.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using YES1 Polyclonal Antibody (Product # PA5-27228). Sample (30 µg of whole cell lysate). Lane A: A431 . 7.5% SDS PAGE. YES1 Polyclonal Antibody (Product # PA5-27228) diluted at 1:1,000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of YES1 was performed by separating 30 µg of various whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a YES1 Polyclonal Antibody (Product # PA5-27228) at a dilution of 1:500.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of c-Yes in methanol-fixed HeLa cells using a c-Yes polyclonal antibody (Product # PA5-27228) at a 1:200 dilution.