PA5-27228

antibody from Invitrogen Antibodies

Targeting: YES1 c-yes, HsT441, Yes

Immunohistochemistry

Antibody data

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Validation data

Product number: PA5-27228 - Provider product page
Provider: Invitrogen Antibodies
Product name: YES1 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Recombinant protein fragment
Description: Recommended positive controls: A431, Neuro 2A, C8D30, NIH-3T3, Raw264.7, PC-12, Rat2. Predicted reactivity: Mouse (99%), Zebrafish (95%), Xenopus laevis (97%), Dog (100%), Chicken (97%), Rhesus Monkey (100%), Bovine (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Isotype: IgG
Vial size: 100 µL
Concentration: 1 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

YES1 protein structure - PA5-27228 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of c-Yes using 30 µg of NIH-3T3 lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a c-Yes polyclonal antibody (Product # PA5-27228) at a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of YES1 was performed by separating 30 µg of various whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a YES1 Polyclonal Antibody (Product # PA5-27228) at a dilution of 1:500.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of YES1 was performed by separating 30 µg of various whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a YES1 Polyclonal Antibody (Product # PA5-27228) at a dilution of 1:500.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using YES1 Polyclonal Antibody (Product # PA5-27228). Sample (30 µg of whole cell lysate). Lane A: A431 . 7.5% SDS PAGE. YES1 Polyclonal Antibody (Product # PA5-27228) diluted at 1:1,000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of YES1 was performed by separating 30 µg of various whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a YES1 Polyclonal Antibody (Product # PA5-27228) at a dilution of 1:500.