sc-17776

antibody from Santa Cruz Biotechnology

Targeting: POLD1 CDC2, POLD

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

sc-17776

Provider

Santa Cruz Biotechnology

Proper citation

Santa Cruz Biotechnology Cat#sc-17776, RRID:AB_675487

Product name

Anti-POLD1

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Reactivity

Human

Host

Mouse

References

Submitted references

Role of the ubiquitin-binding domain of Polη in Rad18-independent translesion DNA synthesis in human cell extracts.

Schmutz V, Janel-Bintz R, Wagner J, Biard D, Shiomi N, Fuchs RP, Cordonnier AM
Nucleic acids research 2010 Oct;38(19):6456-65

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Sequential recruitment of the repair factors during NER: the role of XPG in initiating the resynthesis step.

Mocquet V, Lainé JP, Riedl T, Yajin Z, Lee MY, Egly JM
The EMBO journal 2008 Jan 9;27(1):155-67

Western blot

Supportive validation

Submitted by

per

Main image

Experimental details

Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.

Validation comment

Single band corresponding to the predicted size in kDa (+/-20%).

Primary Ab dilution

1:500

Secondary Ab dilution

1:7000

Lane 1

Marker [kDa]: 230, 110, 82, 49.3, 32.2, 25.5, 17.6

Lane 2

RT-4

Lane 3

U-251MG sp

Lane 4

A-431

Lane 5

Liver

Lane 6

Tonsil

Theoretical target weight

[kDa] 126

Immunohistochemistry

Supportive validation

Submitted by

per

Main image

Experimental details

Immunohistochemical staining of human colon shows strong nuclear positivity in glandular cells.

Validation comment

Staining pattern consistent with experimental and/or bioinformatic data.