Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-34678 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- POLD1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, HeLa, HepG2, Molt-4, Rat2. Predicted reactivity: Mouse (92%), Rat (91%), Bovine (98%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.56 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of POLD1 was performed by separating 30 µg of various whole cell extracts by 5% SDS-PAGE. Proteins were transferred to a membrane and probed with a POLD1 Polyclonal Antibody (Product # PA5-34678) at a dilution of 1:500 and a HRP-conjugated anti-rabbit IgG secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using POLD1 Polyclonal Antibody (Product # PA5-34678). Sample (30 µg of whole cell lysate). Lane A: Molt-4 . 7.5% SDS PAGE. POLD1 Polyclonal Antibody (Product # PA5-34678) diluted at 1:3,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- POLD1 Polyclonal Antibody detects POLD1 protein by Western blot analysis. A. 30 µg Rat2 whole cell lysate/extract.5 % SDS-PAGE. POLD1 Polyclonal Antibody (Product # PA5-34678) dilution: 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of DNA polymerase delta in paraformaldehyde-fixed HeLa cells using a DNA polymerase delta polyclonal antibody (Product # PA5-34678) at a 1:200 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- POLD1 Polyclonal Antibody detects DNA polymerase delta protein at cytoplasm and nucleus on human lung adenocarcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human lung adenocarcinoma. POLD1 Polyclonal Antibody (Product # PA5-34678) diluted at 1:250. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.