Explore
Validate
Learn
Western blot
Immunoprecipitation
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunoprecipitation [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- LS-C674967 - Provider product page
- Provider
- LSBio
- Product name
- POLD1 Antibody LS-C674967
- Antibody type
- Polyclonal
- Description
- Protein G purified
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Storage
- Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
No comments: Submit comment
Enhanced validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western Blot Positive WB detected in: Jurkat whole cell lysate, Hela whole cell lysate, K562 whole cell lysate, A549 whole cell lysate All lanes: POLD1 antibody at 5µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 124 kDa Observed band size: 124 kDa
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Western Blot Positive WB detected in: Jurkat whole cell lysate, Hela whole cell lysate, K562 whole cell lysate, A549 whole cell lysate All lanes: POLD1 antibody at 5µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 124 kDa Observed band size: 124 kDa
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Immunoprecipitating POLD1 in Hela whole cell lysate Lane 1: Rabbit control IgG instead of POLD1 Antibody in Hela whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: POLD1 Antibody (8µg) + Hela whole cell lysate (500µg) Lane 3: Hela whole cell lysate (10µg)
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Immunoprecipitating POLD1 in Hela whole cell lysate Lane 1: Rabbit control IgG instead of POLD1 Antibody in Hela whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: POLD1 Antibody (8µg) + Hela whole cell lysate (500µg) Lane 3: Hela whole cell lysate (10µg)
