A304-007A

antibody from Invitrogen Antibodies

Targeting: POLD1 CDC2, POLD

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

A304-007A

Provider

Invitrogen Antibodies

Product name

PolD1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

4° C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human and mouse PolD1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PolD1 antibody (Product # A304-007A; lot A304-007A-2) used for WB at 0.04 µg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human PolD1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-PolD1 antibody A304-007A (lot A304-007A-1) used for IP at 6 µg/mg lysate. PolD1 was also immunoprecipitated by rabbit anti-PolD1 antibodies A304-005A (albeit inefficiently) and A304-006A. For blotting immunoprecipitated PolD1, A304-007A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human and mouse PolD1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-PolD1 antibody A304-007A (lot A304-007A-1) used for WB at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human PolD1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PolD1 antibody (Product # A304-007A; lot A304-007A-2) used for IP at 6 µg per reaction. PolD1 was also immunoprecipitated by a previous lot of this antibody (lot A304-007A-1). For blotting immunoprecipitated PolD1, Product # A304-007A was used at 1 µg/mL. Detection: Chemiluminescence with an exposure time of 3 seconds.