Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Flow cytometry [1]
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Validation data
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- Product number
- RQ6153 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- RAPGEF3 Antibody / Epac1
- Antibody type
- Polyclonal
- Description
- This highly specific RAPGEF3 antibody is suitable for use in Western blot/Flow cytometry/Direct ELISA applications with human, mouse and rat, monkey samples.
- Reactivity
- Human, Mouse, Rat, Simian
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the RAPGEF3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) human HeLa, 2) human placenta, 3) monkey kidney, 4) rat kidney, 5) rat lung and 6) mouse kidney lysate with RAPGEF3 antibody. Predicted molecular weight ~104 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of human U-87 MG cells with RAPGEF3 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= RAPGEF3 antibody.