GTX129282

antibody from GeneTex

Targeting: ERCC1 RAD10

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX129282

Provider

GeneTex

Product name

ERCC1 antibody

Antibody type

Polyclonal

Reactivity

Human, Rat

Host

Rabbit

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

ERCC1 antibody detects ERCC1 protein by western blot analysis.A. 30 £gg 293T whole cell lysate/extract B. 30 £gg A431 whole cell lysate/extract C. 30 £gg HeLa whole cell lysate/extract D. 30 £gg HepG2 whole cell lysate/extract12 % SDS-PAGEERCC1 antibody (GTX129282) dilution: 1:1000

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

ERCC1 antibody detects ERCC1 protein by western blot analysis.A. 30 £gg 293T whole cell lysate/extract B. 30 £gg whole cell lysate/extract of human ERCC1-transfected 293T cells10 % SDS-PAGEERCC1 antibody (GTX129282) dilution: 1:5000

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

ERCC1 antibody detects ERCC1 protein by western blot analysis.A. 30 £gg PC-12 whole cell lysate/extract B. 30 £gg Rat2 whole cell lysate/extract12 % SDS-PAGEERCC1 antibody (GTX129282) dilution: 1:1000

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Wild-type (WT) and ERCC1 knockout (KO) HeLa cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with ERCC1 antibody (GTX129282) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Wild-type (WT) and ERCC1 knockout (KO) HeLa cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with ERCC1 antibody (GTX129282) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

ERCC1 antibody detects ERCC1 protein at nucleus by immunofluorescent analysis.Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: ERCC1 protein stained by ERCC1 antibody (GTX129282) diluted at 1:1000.Blue: Hoechst 33342 staining.

Immunohistochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

ERCC1 antibody detects ERCC1 protein at cytoplasm and nucleus on human lung carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human lung carcinoma. ERCC1 antibody (GTX129282) diluted at 1:250.