Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [5]
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Validation data
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- Product number
- NBP2-34480 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal ERCC1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A purified. Specificity of human ERCC1 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human
- Host
- Mouse
- Antigen sequence
KARRLFDVLH
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: ERCC1 Antibody (CL1284) [NBP2-34480] - Simple Western lane view shows a specific band for ADARB1 in 0.2 mg/ml of RT-4, U-251MG sp lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. *Non-specific interaction with the 230 kDa standard may be seen with this antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ERCC1 Antibody (CL1284) [NBP2-34480] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-ERCC1 antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ERCC1 Antibody (CL1284) [NBP2-34480] - Lane 1: Marker [kDa] Lane 2: Human cell line RT-4
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: ERCC1 Antibody (CL1284) [NBP2-34480] - Staining of human colon shows nuclear immunoreactivity in both glandular and lymphoid cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: ERCC1 Antibody (CL1284) [NBP2-34480] - Staining of human colorectal cancer shows strong nuclear immunoreactivity in tumor cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: ERCC1 Antibody (CL1284) [NBP2-34480] - Staining of human prostate cancer shows strong nuclear immunoreactivity in tumor cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: ERCC1 Antibody (CL1284) [NBP2-34480] - Staining of human ventricular cancer shows strong nuclear positivity in tumor cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: ERCC1 Antibody (CL1284) [NBP2-34480] - Staining of human testis shows strong nuclear positivity in seminiferous tubules.