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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [5]
- Flow cytometry [2]
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Validation data
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- Product number
- GTX84547 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX84547, RRID:AB_10730538
- Product name
- ERCC1 antibody [2H5]
- Antibody type
- Monoclonal
- Reactivity
- Human
- Host
- Mouse
- Storage
- For short-term storage, store at 4¢XC or aliquot into working amounts and store at -20¢XC. For long-term storage, store at -70¢XC (aliquotted). Avoid repeated freeze-thaw cycles.
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Supportive validation
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- GeneTex (provider)
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- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ERCC1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ERCC1.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Anti-ERCC1 mouse monoclonal antibody (GTX84547) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ERCC1.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded human colon tissue using anti-ERCC1 mouse monoclonal antibody. (GTX84547, Dilution 1:50)
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded human kidney tissue using anti-ERCC1 mouse monoclonal antibody. (GTX84547, Dilution 1:50)
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded adenocarcinoma of human ovary tissue using anti-ERCC1 mouse monoclonal antibody. (GTX84547, Dilution 1:50)
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded human pancreas tissue using anti-ERCC1 mouse monoclonal antibody. (GTX84547, Dilution 1:50)
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded human prostate tissue using anti-ERCC1 mouse monoclonal antibody. (GTX84547, Dilution 1:50)
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Flow cytometric analysis of Hela cells, using anti-ERCC1 antibody (GTX84547), (Red), compared to a nonspecific negative control antibody (Blue).
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Flow cytometric analysis of Jurkat cells, using anti-ERCC1 antibody (GTX84547), (Red), compared to a nonspecific negative control antibody (Blue).
