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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Flow cytometry [2]
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Validation data
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- Product number
- TA501184 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA501184, RRID:AB_11125884
- Product name
- ERCC1 mouse monoclonal antibody, clone OTI1H1 (formerly 1H1)
- Antibody type
- Monoclonal
- Description
- ERCC1 mouse monoclonal antibody, clone OTI1H1 (formerly 1H1)
- Reactivity
- Canine
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- ERCC1
- Isotype
- IgG
- Antibody clone number
- OTI1H1
- Vial size
- 100 µl
- Concentration
- 0.81 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ERCC1 (RC200478, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ERCC1.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ERCC1 monoclonal antibody.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-ERCC1 mouse monoclonal antibody (TA501184) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ERCC1(RC200478).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometric Analysis of Jurkat cells, using anti-ERCC1 antibody(TA501184),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
- Validation comment
- FC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either RC200478 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-ERCC1 antibody(TA501184), and then analyzed by flow cytometry.
- Validation comment
- FC
