TA504286

antibody from OriGene

Targeting: ERCC1 RAD10

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA504286

Provider

OriGene

Proper citation

OriGene Cat#TA504286, RRID:AB_11124225

Product name

ERCC1 mouse monoclonal antibody, clone OTI24F2 (formerly 24F2)

Antibody type

Monoclonal

Description

ERCC1 mouse monoclonal antibody, clone OTI24F2 (formerly 24F2)

Host

Mouse

Conjugate

Unconjugated

Epitope

ERCC1

Isotype

IgG

Antibody clone number

OTI24F2

Vial size

100 µl

Concentration

1.21 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ERCC1 (RC200478, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ERCC1.

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Anti-ERCC1 mouse monoclonal antibody (TA504286) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ERCC1(RC200478).

Validation comment

IF

Flow cytometry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells transfected with either RC200478 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-ERCC1 antibody(TA504286), and then analyzed by flow cytometry.

Validation comment

FC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Flow cytometric Analysis of Hela cells, using anti-ERCC1 antibody(TA504286),(Red), compared to a nonspecific negative control antibody,(Blue).

Validation comment

FC