AF-273-NA

antibody from R&D Systems

Targeting: FGF9

Western blot (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AF-273-NA

Provider

R&D Systems

Product name

Human FGF-9 Antibody

Antibody type

Polyclonal

Description

Antigen Affinity-purified. Detects human FGF-9 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) FGF-4, rhFGF-5, rhFGF-6, rhFGF-7, rhFGF-8, rhFGF-10, rhFGF-17, rhFGF-18, and rhFGF-19 is observed.

Reactivity

Human

Host

Goat

Conjugate

Unconjugated

Antigen sequence

P31371


Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

Fibroblast Growth Factor 9 Imparts Hierarchy and Vasoreactivity to the Microcirculation of Renal Tumors and Suppresses Metastases.

Yin H, Frontini MJ, Arpino JM, Nong Z, O'Neil C, Xu Y, Balint B, Ward AD, Chakrabarti S, Ellis CG, Gros R, Pickering JG
The Journal of biological chemistry 2015 Sep 4;290(36):22127-42

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Relationship between the localization of fibroblast growth factor 9 in prostate cancer cells and postoperative recurrence.

Teishima J, Shoji K, Hayashi T, Miyamoto K, Ohara S, Matsubara A
Prostate cancer and prostatic diseases 2012 Mar;15(1):8-14

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Fibroblast growth factor 9 delivery during angiogenesis produces durable, vasoresponsive microvessels wrapped by smooth muscle cells.

Frontini MJ, Nong Z, Gros R, Drangova M, O'Neil C, Rahman MN, Akawi O, Yin H, Ellis CG, Pickering JG
Nature biotechnology 2011 May;29(5):421-7

Immunohistochemistry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

FGF-9 in Human Placenta. FGF-9 was detected in immersion fixed paraffin-embedded sections of human placenta using 5 µg/mL Goat Anti-Human FGF-9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-273-NA) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-AEC Cell & Tissue Staining Kit (red; Catalog # CTS009) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.