- PA5-81175 - Invitrogen Antibodies FOSL1 antibody

Submitted references The Transcription Factor FRA-1/AP-1 Controls Lipocalin-2 Expression and Inflammation in Sepsis Model.

Cao S, Schnelzer A, Hannemann N, Schett G, Soulat D, Bozec A
Frontiers in immunology 2021;12:701675

No comments: Submit comment

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of Fra1 in Lane A: HepG2 Whole Cell Lysate (30 µg), Lane B: Jurkat Whole Cell Lysate (30 µg) . Samples were probed using a Fra1 Polyclonal Antibody (Product # PA5-81175) at a 1:500 dilution, followed by a Goat Anti-Rabbit IgG (H+L), Dylight 800 Secondary Antibody at a 1:10000 dilution. Western blot was performed under reducing conditions. Predicted band size:29 kDa. Observed band size:37 kDa.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using Fra1 Polyclonal Antibody (Product # PA5-81175) at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate, Lane B: Jurkat Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG H&L (DyLight™ 800) at 1:10,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 29 kDa. Observed band size: 37 kDa.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence staining of FOSL1 in A431 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Fra1 Polyclonal Antibody (Product # PA5-81175) (1 µg/mL) at 4°C overnight. Then cells were stained with the Alexa Fluor®594-conjugated Goat Anti-rabbit IgG secondary antibody (red). Positive staining was localized to nucleus.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fra1 Immunoprecipitation using: Lane A: 0.5 mg HepG2 Whole Cell Lysate, Lane B: 0.5 mg Jurkat Whole Cell Lysate 0.5 µL with Fra1 Polyclonal Antibody (Product # PA5-81175) and 60 μg of Immunomagnetic beads Protein G. Primary antibody: Fra1 Polyclonal Antibody, at 1:500 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 29 kDa. Observed band size: 26 kDa.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 3 Increased expression of Lcn2 after induction of endotoxin tolerance is reduced in Fosl1 knockout murine macrophages. (A) Quantitative RT-PCR analysis of AP-1 transcription factor gene expression in littermate control and Fra-1 deficient BMDM after LPS stimulation in both naive and tolerant state, normalized to the expression of Actb . (B) Western blot detection of FRA-1 protein after LPS stimulation in littermate control and FRA-1 deficient BMDM in both naive and tolerant state. GAPDH was used as loading control. (C) Quantitative RT-PCR analysis of Il6 and Lcn2 in littermate control and FRA-1 deficient BMDM after LPS stimulation in both naive and tolerant state. Left panels represent the kinetic of gene expression normalized to the expression of Actb and right panels represent the area under curve (AUC) for the respective genes. (D) IL-6 and NGAL protein levels in the supernatant of wild type and FRA-1 deficient BMDM after LPS stimulation in both naive and tolerant state. Left panels represent the kinetic of protein secretion and right panels represent the area under curve (AUC) for the respective protein. The data shown are from one experiment representative of three independent biological replicates and they are shown as mean +- s.e.m. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001 by two-way RM ANOVA with Tukey's multiple comparisons test. Black asterisks indicated significant difference between naive (n=6) versus tolerant (n=9) state in littermate control mi

PA5-81175

Antibody data