Antibody data
- Antibody Data
 - Antigen structure
 - References [1]
 - Comments [0]
 - Validations
 - Western blot [1]
 - Immunocytochemistry [1]
 - Immunohistochemistry [1]
 
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Validation data
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- Product number
 - TA307406 - Provider product page

 - Provider
 - OriGene
 - Product name
 - Rabbit monoclonal antibody against c-Rel(clone EPR2558)
 - Antibody type
 - Monoclonal
 - Description
 - Rabbit monoclonal antibody against c-Rel(clone EPR2558)
 - Host
 - Rabbit
 - Conjugate
 - Unconjugated
 - Epitope
 - REL
 - Isotype
 - IgG
 - Antibody clone number
 - EPR2558
 - Vial size
 - 100 µl
 - Concentration
 - NULL
 
Submitted references		NFkB is activated by multiple mechanisms in hairy cell leukemia.
				
		
	
			Nagel S, Ehrentraut S, Meyer C, Kaufmann M, Drexler HG, MacLeod RA
Genes, chromosomes & cancer 2015 Jul;54(7):418-32
		Genes, chromosomes & cancer 2015 Jul;54(7):418-32
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					Supportive validation
					
									
				
				- Submitted by
 - OriGene (provider)
 - Main image
 
- Experimental details
 - Western blot - c-Rel antibody [EPR2558]; All lanes : Anti-c-Rel antibody [EPR2558] at 1/1000 dilution.Lane 1 : Daudi cell lysate.Lane 2 : HuT-78 cell lysate.Lane 3 : K562 cell lysate.Lysates/proteins at 10 ug per lane.Predicted band size : 69 kDa.
 - Validation comment
 - WB
 
							
					Supportive validation
					
									
				
				- Submitted by
 - OriGene (provider)
 - Main image
 
- Experimental details
 - Immunocytochemistry/ Immunofluorescence - Anti-c-Rel antibody [EPR2558]; TA307406 staining c-Rel in human macrophages by Immunocytochemistry/ Immunofluorescence.Cells were fixed in paraformaldehyde, permeabilized using Triton X-100, blocked with 5% goat serum for 1 hour at 37?°C and then incubated with TA307406 at a 1/500 dilution for 3 hours at 37?°C. The secondary used was an Alexa-Fluor 488 conjugated goat anti-rabbit monoclonal used at a 1/1000 dilution.
 - Validation comment
 - IF
 
							
					Supportive validation
					
									
				
		- Submitted by
 - OriGene (provider)
 - Main image
 
- Experimental details
 - Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - c-Rel antibody [EPR2558]; Immunohistochemical analysis of Human diffuse B-cell lymphoma tissue using TA307406 at a dilution of 1/100.
 - Validation comment
 - IHC