Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- TA307406 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit monoclonal antibody against c-Rel(clone EPR2558)
- Antibody type
- Monoclonal
- Description
- Rabbit monoclonal antibody against c-Rel(clone EPR2558)
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- REL
- Isotype
- IgG
- Antibody clone number
- EPR2558
- Vial size
- 100 µl
- Concentration
- NULL
Submitted references NFkB is activated by multiple mechanisms in hairy cell leukemia.
Nagel S, Ehrentraut S, Meyer C, Kaufmann M, Drexler HG, MacLeod RA
Genes, chromosomes & cancer 2015 Jul;54(7):418-32
Genes, chromosomes & cancer 2015 Jul;54(7):418-32
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot - c-Rel antibody [EPR2558]; All lanes : Anti-c-Rel antibody [EPR2558] at 1/1000 dilution.Lane 1 : Daudi cell lysate.Lane 2 : HuT-78 cell lysate.Lane 3 : K562 cell lysate.Lysates/proteins at 10 ug per lane.Predicted band size : 69 kDa.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunocytochemistry/ Immunofluorescence - Anti-c-Rel antibody [EPR2558]; TA307406 staining c-Rel in human macrophages by Immunocytochemistry/ Immunofluorescence.Cells were fixed in paraformaldehyde, permeabilized using Triton X-100, blocked with 5% goat serum for 1 hour at 37?°C and then incubated with TA307406 at a 1/500 dilution for 3 hours at 37?°C. The secondary used was an Alexa-Fluor 488 conjugated goat anti-rabbit monoclonal used at a 1/1000 dilution.
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - c-Rel antibody [EPR2558]; Immunohistochemical analysis of Human diffuse B-cell lymphoma tissue using TA307406 at a dilution of 1/100.
- Validation comment
- IHC