655803

antibody from BioLegend

Targeting: REL c-Rel, I-Rel

Western blot (Data presented on provider website)

Chromatin Immunoprecipitation (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

655803

Provider

BioLegend

Proper citation

BioLegend Cat#655803, RRID:AB_2687119

Product name

Go-ChIP-Grade™ Purified anti-c-REL

Antibody type

Monoclonal

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Antibody clone number

2F5A22

Vial size

25 µg

Concentration

0.5 mg/ml

Storage

2°C-8°C

Handling

Ambient RT

Other assay

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

Chromatin Immunoprecipitation (ChIP) was performed using commercial Protein-G coated 96 well high-throughput ChIP assay kit by loading 3µg of cross-linked chromatin samples from Jurkat cells treated with TPA and Ionomycin with either A) 1:50 dilution of Go-ChIP-Grade™ Purified anti-c-REL (Clone 2F5A22), B) equal amount of Purified Mouse IgG2b, κ Isotype Control Antibody, or C) competitor’s ChIP-grade Purified anti-c-REL Antibody and D) equal amount of matched Isotype Control Antibody as recommended by the manufacturer. The enriched DNA was purified and quantified by real-time qPCR using primers targeting human Bcl2L1 gene region and human αSatellite repeat (as a negative control). The amount of immunoprecipitated DNA in each sample is represented as signal relative to the 5% of total amount of input chromatin.

Conjugate

Unconjugated