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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- PAB10284 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB10284, RRID:AB_1712813
- Product name
- REL polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of REL.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
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Supportive validation
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- Abnova Corporation (provider)
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- Experimental details
- Immunoblot of HeLa cell extract.All incubations except color development were performed using TBS supplemented with 0.1% Tween-20 at room temperature.The membrane was blocked in 5% dry milk for 2 h.After washing, a 1 : 1,000 dilution of the primary antibody was added to the membrane and incubated for 2 h.Washes with buffer were performed 4 times for 5' each.The immunoblot was incubated with secondary antibody (HRP Goat-a-RabbitIgG [H&L]) diluted 1:2,000 for 1h.Washes with TBS precededcolor development.
