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Western blot
Gel shiftAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- GTX19464 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX19464, RRID:AB_423590
- Product name
- NFkB c Rel antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide (Human) conjugated to KLH (C terminal).
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 50µl
- Concentration
- Batch dependent
- Storage
- Keep as concentrated solution. Store at 4°C short term. For extended storage aliquot and store at -20°C or below. Avoid freeze-thaw cycles.
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Supportive validation
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- GeneTex (provider)
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- Experimental details
- Western blot of HeLa cell extract. All incubations except color development were performed using TBS supplemented with 0.1% Tween-20 at room temperature. The membrane was blocked in 5% dry milk for 2 h. After washing, a:1:1,000 dilution of the primary antibody (GTX19464) was added to the membrane and incubated for 2 h. Washes with buffer were performed 4 times for 5' each. The western blot was incubated with secondary antibody (HRP Goat-a-Rabbit IgG [H&L]) diluted 1:2,000 for 1 h. Washes with TBS preceded color development.
- Validation comment
- WB
