Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-19277 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- c-Rel Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with canine based on sequence homology. This antibody is tested in Peptide ELISA: antibody detection limit dilution 16,000.
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot staining of Human Spleen lysate using Product # PA5-19277 at a concentration of 0.5 µg/mL, the primary antibody incubation was 1 hour and the detection method was chemiluminescence.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using anti-c-REL Polyclonal Antibody(Product # PA5-19277) and 68 kDa band corresponding to c-REL was observed across tissues except in Mouse and Rat Heart. An additional uncharacterized bands at ~ 55 kDa was observed across the tissues tested. Tissue extracts of (30 µg lysate) of Mouse Thymus (Lane 1), Mouse Spleen (Lane 2), Rat Thymus (Lane 3), Rat Spleen (Lane 4), Mouse Heart (Lane 5) and Rat Heart (Lane 6) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (0.5 µg/mL) and detected by Rabbit anti-Goat IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27014, 1:1000 dilution). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of c-Rel in Human Thymus using a c-Rel monoclonal antibody (Product #PA5-19277) at 2.5 µg/mL. The Human Thymus tissue section was paraffin embeded and detected using steamed antigen retrieval with citrate buffer pH 6, AP-staining.