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- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- MA5-15859 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- c-Rel Monoclonal Antibody (1E7)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Description
- MA5-15859 targets c-Rel in indirect ELISA, IF and IHC applications and shows reactivity with Human and mouse samples. MA5-15859 targets c-Rel in WB against recombinant c-Rel and transfected cell lysates only. The MA5-15859 immunogen is purified recombinant fragment of human c-Rel expressed in E. Coli. MA5-15859 detects c-Rel which has a predicted molecular weight of approximately 78 kDa.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 1E7
- Vial size
- 100 µL
- Concentration
- Conc. Not Determined
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Single-Cell RNA-Sequencing Reveals Lineage-Specific Regulatory Changes of Fibroblasts and Vascular Endothelial Cells in Keloids.
Liu X, Chen W, Zeng Q, Ma B, Li Z, Meng T, Chen J, Yu N, Zhou Z, Long X
The Journal of investigative dermatology 2022 Jan;142(1):124-135.e11
The Journal of investigative dermatology 2022 Jan;142(1):124-135.e11
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of c-Rel using c-Rel monoclonal antibody (Product # MA5-15859) in Jurkat (1), NIH/3T3 (2), HeLa (3), HEK293 (4) and RAJI (5) cell lysate.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis using c-Rel Monoclonal Antibody (1E7) (Product # MA5-15859) against human c-Rel (AA: 13-139) recombinant protein. (Expected MW is 40.2 kDa)
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis using c-Rel Monoclonal Antibody (1E7) (Product # MA5-15859) against HEK293-6e (Lane 1) and c-Rel (AA: 13-139)-hIgGFc transfected HEK293-6e (Lane 2) cell lysate.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of U251 cells using c-Rel monoclonal antibody (Product # MA5-15859) (Green). Blue: DRAQ5 fluorescent DNA dye. Red: actin filaments have been labeled with phalloidin.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded endometrial cancer tissues (left) and liver cancer tissues (right) using c-Rel monoclonal antibody (Product # MA5-15859) followed with DAB staining.
