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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- NB600-474 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB600-474, RRID:AB_10002651
- Product name
- Rabbit Polyclonal c-Rel Antibody
- Antibody type
- Polyclonal
- Description
- Delipidation and Defibrination. May react non specifically with other proteins.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Vial size
- 0.1 ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Western Blot: c-Rel Antibody [NB600-474] - All incubations except color development were performed using TBS supplemented with 0.1% Tween-20 at room temperature. The membrane was blocked in 5% dry milk for 2 h. After washing, a:1:1,000 dilution of the primary antibody was added to the membrane and incubated for 2 h. Washes with buffer were performed 4 times for 5' each. The western blot was incubated with secondary antibody (HRP Goat-a-Rabbit IgG [H&L]) diluted 1:2,000 for 1 h. Washes with TBS preceded color development.
