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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Other assay [1]
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- Product number
- PA1-84572 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GAD65/GAD67 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- A suggested positive control for immunohistochemical applications is brain. In Western blot, a doublet at ~65/67 kDa has been observed. Rabbit anti Glutamate Decarboxylase 1/2 antibody recognizes both molecular forms of glutamate decarboxylase, GAD1 (GAD67) and GAD2 (GAD65), the enzyme which converts glutamic acid to gamma -aminobutyric acid (GABA), the major inhibitory transmitter in the higher brain region.
- Reactivity
- Human, Mouse, Rat, Feline
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-GAD65/GAD67 Polyclonal Antibody (Product # PA1-84572) and a 60 kDa band corresponding to GAD65/GAD67 was observed across tested samples. Whole cell extracts (40 µg lysate) of Mouse Brain (Lane 1), Rat Brain (Lane 2), Mouse Kidney (Lane 3), Rat Kidney (Lane 4) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036,1:20000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Pico PLUS Chemiluminescent Substrate (Product # 34580).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 4 FIGURE Decrease in the synaptic localization of inhibitory synaptic markers in Tg-FDD mice. (A) Immunofluorescence for GABA A receptor (green) and GAD (red) on cortex and hippocampus. (B) Quantification of the number of merge puncta for both markers on A. (C) Double staining for GABA B receptor (green) and GAD (red). (D) Quantification of the number of merged puncta for both markers on C. p < 0.05 indicated on each graph, Mann-Whitney test, n = 9 photographs from 3 different animals per genotype. Data represented as mean +- SEM. Scale bar 10 mum.
