Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Immunocytochemistry [2]
- Immunohistochemistry [10]
- Other assay [1]
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Validation data
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- Product number
- MA5-24909 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GAD67 Monoclonal Antibody (OTI3G9)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- OTI3G9
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20°C, Avoid Freeze/Thaw Cycles
Submitted references Deep brain stimulation of the anterior nuclei of the thalamus relieves basal ganglia dysfunction in monkeys with temporal lobe epilepsy.
Du T, Chen Y, Shi L, Liu D, Liu Y, Yuan T, Zhang X, Zhu G, Zhang J
CNS neuroscience & therapeutics 2021 Mar;27(3):341-351
CNS neuroscience & therapeutics 2021 Mar;27(3):341-351
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of GAD1 in A549 cells. Cells were probed with a GAD1 monoclonal antibody (Product # MA5-24909).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of GAD1 in A549 cells. Cells were probed with a GAD1 monoclonal antibody (Product # MA5-24909).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry was performed on paraffin-embedded adenocarcinoma of human ovary tissue. To expose target proteins, 10mM citric buffer, pH6.0, 100°C for 10min was used. Following antigen retrieval, tissues were probed with a GAD1 monoclonal antibody (Product # MA5-24909).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry was performed on paraffin-embedded human pancreas tissue. To expose target proteins, 10mM citric buffer, pH6.0, 100°C for 10min was used. Following antigen retrieval, tissues were probed with a GAD1 monoclonal antibody (Product # MA5-24909).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry was performed on paraffin-embedded human endometrium tissue. To expose target proteins, 10mM citric buffer, pH6.0, 100°C for 10min was used. Following antigen retrieval, tissues were probed with a GAD1 monoclonal antibody (Product # MA5-24909).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry was performed on paraffin-embedded adenocarcinoma of human breast tissue. To expose target proteins, 10mM citric buffer, pH6.0, 100°C for 10min was used. Following antigen retrieval, tissues were probed with a GAD1 monoclonal antibody (Product # MA5-24909).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry was performed on paraffin-embedded human thyroid tissue. To expose target proteins, 10mM citric buffer, pH6.0, 100°C for 10min was used. Following antigen retrieval, tissues were probed with a GAD1 monoclonal antibody (Product # MA5-24909).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry was performed on paraffin-embedded carcinoma of human kidney tissue. To expose target proteins, 10mM citric buffer, pH6.0, 100°C for 10min was used. Following antigen retrieval, tissues were probed with a GAD1 monoclonal antibody (Product # MA5-24909).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry was performed on paraffin-embedded human lung tissue. To expose target proteins, 10mM citric buffer, pH6.0, 100°C for 10min was used. Following antigen retrieval, tissues were probed with a GAD1 monoclonal antibody (Product # MA5-24909).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry was performed on paraffin-embedded human colon tissue. To expose target proteins, 10mM citric buffer, pH6.0, 100°C for 10min was used. Following antigen retrieval, tissues were probed with a GAD1 monoclonal antibody (Product # MA5-24909).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry was performed on paraffin-embedded human ovary tissue. To expose target proteins, 10mM citric buffer, pH6.0, 100°C for 10min was used. Following antigen retrieval, tissues were probed with a GAD1 monoclonal antibody (Product # MA5-24909).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry was performed on paraffin-embedded carcinoma of human bladder tissue. To expose target proteins, 10mM citric buffer, pH6.0, 100°C for 10min was used. Following antigen retrieval, tissues were probed with a GAD1 monoclonal antibody (Product # MA5-24909).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- FIGURE 5 A, Analysis of GAD67 in the GPi via Western blotting. B, Expression of GAD67 was high in the GPi of the EP and EP-sham-DBS groups. A significant decline in GAD67 was observed in the TLE monkeys stimulated with ANT-DBS in the EP-DBS group. C, Immunofluorescent staining of GAD67 and NeuN in GPi. D, Immunofluorescent intensity of GAD67 increased in the TLE model, but was reversed by ANT-DBS. * P < .05; ** P < .01; *** P < .001. DBS, deep brain stimulation; ANT, anterior nuclei of the thalamus; EP, epilepsy; GAD67, glutamate decarboxylase 67 [Colour figure can be viewed at wileyonlinelibrary.com ]